製品の概要
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製品名Anti-POLR3B antibody
POLR3B 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to POLR3B
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由来種Rabbit
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アプリケーション適用あり: WB, IP, IHC-Pmore details
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種交差性交差種: Human
交差が予測される動物種: Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus, Elephant
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免疫原
A region between residue 1083 and 1133 of human POLR3B using the numbering given in entry NP_060552.3.
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ポジティブ･コントロール
- HeLa nuclear lysate 293T nuclear lysate
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーPreservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab86143 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 128 kDa. | |
| IP | Use at 2-5 µg/mg of lysate. | |
| IHC-P | 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol III is composed of mobile elements and RPC2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft (By similarity). Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway.
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配列類似性Belongs to the RNA polymerase beta chain family.
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細胞内局在Nucleus.
- Information by UniProt
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参照データベース
- Entrez Gene: 55703 Human
- SwissProt: Q9NW08 Human
- Unigene: 610795 Human
- Unigene: 62696 Human
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別名
- C128 antibody
- DNA directed RNA polymerase III 127.6 kDa polypeptide antibody
- DNA directed RNA polymerase III subunit B antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLR3B antibody (ab86143)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling POLR3B with ab86143 at 1/1000 (0.2µg/ml). Detection: DAB. -
Western blot - Anti-POLR3B antibody (ab86143)All lanes : Anti-POLR3B antibody (ab86143) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 128 kDa
Observed band size: 128 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-POLR3B antibody (ab86143)Detection of Human POLR3B by Immunoprecipitation, using ab86143 at 3µg/mg lysate. Image shows immunoprecipitated POLR3B detected with post IP WB, loading 20% of IP and using HeLa whole cell lysate at 1mg, with an antibody recognising an upsatream eptiope in lane 1, ab86143 at 1 µg/ml in lane 2 and control IgG in lane 3. Detection: Chemiluminescence with an exposure time of 30 seconds.
参考文献
ab86143 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"