製品の概要

  • 製品名Anti-POLR3A antibody - ChIP Grade
    POLR3A 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to POLR3A - ChIP Grade
  • アプリケーション適用あり: WB, ChIPmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Cow
  • 免疫原

    Recombinant protein fragment contain a sequence corresponding to a region within amino acids 182 and 452 of POLR3A

  • ポジティブ・コントロール
    • HCT116 whole cell lysate

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab96328 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 156 kDa.
ChIP Use at an assay dependent concentration.

ターゲット情報

  • 機能DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (By similarity). Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway.
  • 組織特異性Expressed in the brain, in the cortex and the white matter (at protein level).
  • 関連疾患Leukodystrophy, hypomyelinating, 7, with or without oligodontia and/or hypogonadotropic hypogonadism
  • 配列類似性Belongs to the RNA polymerase beta' chain family.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BC053071 antibody
    • DNA directed RNA polymerase III largest subunit antibody
    • DNA directed RNA polymerase III subunit A antibody
    • DNA-directed RNA polymerase III largest subunit antibody
    • DNA-directed RNA polymerase III subunit A antibody
    • DNA-directed RNA polymerase III subunit RPC1 antibody
    • hRPC155 antibody
    • MGC62420 antibody
    • POLR 3A antibody
    • POLR3A antibody
    • Polymerase (RNA) III (DNA directed) polypeptide A 155kDa antibody
    • Polymerase (RNA) III (DNA directed) polypeptide A antibody
    • RGD1305574 antibody
    • RNA polymerase III 155 kDa subunit antibody
    • RNA polymerase III subunit C1 antibody
    • RNA polymerase III subunit C160 antibody
    • RNA polymerase III subunit RPC155 D antibody
    • RPC1 antibody
    • RPC1_HUMAN antibody
    • RPC155 antibody
    see all

Anti-POLR3A antibody - ChIP Grade 画像

  • ChIP analysis using ab96328 binding POLR3A in 3T3 fibroblast whole cell lysate. Cells were cross-linked for 10 minutes with formaldehyde. Samples were incubated with undiluted primary antibody for 12 hours at 4°C. Protein binding was detected using real-time PCR.
    Positive control: tRNA le and tRNA ser.
    Negative Control:IgG.

    See Abreview

  • Anti-POLR3A antibody - ChIP Grade (ab96328) at 1/500 dilution + HCT116 cell lysate at 30 µg

    Predicted band size : 156 kDa

Anti-POLR3A antibody - ChIP Grade (ab96328) 使用論文

This product has been referenced in:

See 1 Publication for this product

Product Wall

Application ChIP
Detection step Real-time PCR
Sample Mouse Cell lysate - whole cell (3T3 Fibroblasts)
Specification 3T3 Fibroblasts
Negative control IgG
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Positive control tRNA leu & tRNA ser
Username

Ms. Jennifer Blancas

Verified customer

投稿 Jan 20 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ChIP
Sample Human Cell lysate - whole cell (Raji)
Specification Raji
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Detection step Real-time PCR
Positive control Primer set for tRNA-Leu 5'-GTC AGG ATG GCC GAG TCT AAG-3' and 5'-CCA CGC CTC CAT ACG GAG AAC CAG AAG ACC C-3' from Johnson, S.S. et al Molecular Cell
Negative control IgG and primer set for Alu on Chromosome 10 5’GATTCTCAACAGCAGAATTCCATGCC3’ and 5’CATGTTTGAGAATGTCTACTTCTTAG3’ from Zeng, W. et al 2009 PLOS Genetics
Username

Abcam user community

Verified customer

投稿 Jan 16 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"