Anti-POLR3A 抗体 (ab96328)
Key features and details
- Rabbit polyclonal to POLR3A
- Suitable for: ChIP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-POLR3A antibody
POLR3A 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to POLR3A -
由来種
Rabbit -
アプリケーション
適用あり: ChIP, WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Cow -
免疫原
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 182 and 452 of POLR3A
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab96328の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ChIP | (2) |
Use at an assay dependent concentration.
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WB |
1/500 - 1/3000. Predicted molecular weight: 156 kDa.
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特記事項 |
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ChIP
Use at an assay dependent concentration. |
WB
1/500 - 1/3000. Predicted molecular weight: 156 kDa. |
ターゲット情報
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機能
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (By similarity). Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. -
組織特異性
Expressed in the brain, in the cortex and the white matter (at protein level). -
関連疾患
Leukodystrophy, hypomyelinating, 7, with or without oligodontia and/or hypogonadotropic hypogonadism -
配列類似性
Belongs to the RNA polymerase beta' chain family. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 11128 Human
- Entrez Gene: 218832 Mouse
- Omim: 614258 Human
- SwissProt: O14802 Human
- SwissProt: B2RXC6 Mouse
- Unigene: 436896 Human
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別名
- BC053071 antibody
- DNA directed RNA polymerase III largest subunit antibody
- DNA directed RNA polymerase III subunit A antibody
see all
画像
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (14)
ab96328 は 14 報の論文で使用されています。
- Van Bortle K et al. A cancer-associated RNA polymerase III identity drives robust transcription and expression of snaR-A noncoding RNA. Nat Commun 13:3007 (2022). PubMed: 35637192
- Chen L et al. 5' Half of specific tRNAs feeds back to promote corresponding tRNA gene transcription in vertebrate embryos. Sci Adv 7:eabh0494 (2021). PubMed: 34797706
- Zhao J et al. APOE4 exacerbates synapse loss and neurodegeneration in Alzheimer's disease patient iPSC-derived cerebral organoids. Nat Commun 11:5540 (2020). PubMed: 33139712
- Tucker JM et al. Alteration of the Premature tRNA Landscape by Gammaherpesvirus Infection. mBio 11:N/A (2020). PubMed: 33323507
- Ramsay EP et al. Structure of human RNA polymerase III. Nat Commun 11:6409 (2020). PubMed: 33335104