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Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human POLDIP2.
(Peptide available as ab99870.)
Our Abpromise guarantee covers the use of ab68663 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).|
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: POLDIP2 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: HepG2 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab68663 observed at 40 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab68663 was shown to specifically recognize POLDIP2 in wild-type HAP1 cells along with additional cross reactive bands. No band was observed when POLDIP2 knockout cells were examined. Wild-type and POLDIP2 knockout samples were subjected to SDS-PAGE. ab68663 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1 µg/ml and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.
ab68663 has not yet been referenced specifically in any publications.