製品の概要

  • 製品名Anti-POLDIP1 antibody
    POLDIP1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to POLDIP1
  • アプリケーション適用あり: ELISA, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide derived from a region between amino acids 26-75 of Human POLDIP1. Note: The exact sequence is proprietary.

  • ポジティブ・コントロール
    • WB: Fetal heart lysate. IHC-P: Human brain tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab32974 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/1.5625e+006.
WB Use a concentration of 0.25 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 36 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex involved in regulation of cytoskeleton structure. The BCR(BACURD1) E3 ubiquitin ligase complex mediates the ubiquitination of RHOA, leading to its degradation by the proteasome, thereby regulating the actin cytoskeleton and cell migration.
  • 組織特異性Expressed in a wide variety of tissues.
  • パスウェイProtein modification; protein ubiquitination.
  • 配列類似性Belongs to the BACURD family.
    Contains 1 BTB (POZ) domain.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BACD1_HUMAN antibody
    • BACURD1 antibody
    • BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 1 antibody
    • BTB/POZ domain-containing protein KCTD13 antibody
    • FKSG86 antibody
    • hBACURD1 antibody
    • KCTD13 antibody
    • PDIP1 antibody
    • POLDIP 1 antibody
    • POLDIP1 antibody
    • Polymerase delta-interacting protein 1 antibody
    • Potassium channel tetramerisation domain containing 13 antibody
    • PP6832 antibody
    • TNFAIP1 like polymerase delta interacting protein 1 antibody
    • TNFAIP1-like protein antibody
    see all

Anti-POLDIP1 antibody 画像

  • Anti-POLDIP1 antibody (ab32974) at 0.25 µg/ml + fetal heart lysate

    Secondary
    HRP conjugated anti Rabbit IgG 1/50000 - 1/100000

    Predicted band size : 36 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue labelling POLDIP1 with ab32974 at 4-8µg/ml. Cells with positive label (indicated by arrows) are neural cells. Magnification: 400X.

Anti-POLDIP1 antibody (ab32974) 使用論文

This product has been referenced in:
  • Werwein E  et al. B-Myb promotes S-phase independently of its sequence-specific DNA binding activity and interacts with polymerase delta-interacting protein 1 (Pdip1). Cell Cycle 11:4047-58 (2012). Read more (PubMed: 23032261) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"