製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to POGZ
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide: ADTDLFMECE EEELEPWQKI SDVIEDSVVE DYNSVDKTTT VSVSQQPVSA , corresponding to amino acids 2-51 of Human POGZ

  • ポジティブ・コントロール
    • Brain lysate, human lung tissue and human kidney tissue.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab66652 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 5 µg/ml. Detects a band of approximately >90 kDa (predicted molecular weight: 155 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

ターゲット情報

  • 機能
    Plays a role in mitotic cell cycle progression and is involved in kinetochore assembly and mitotic sister chromatid cohesion. Probably through its association with CBX5 plays a role in mitotic chromosome segregation by regulating aurora kinase B/AURKB activation and AURKB and CBX5 dissociation from chromosome arms.
  • 配列類似性
    Contains 9 C2H2-type zinc fingers.
    Contains 1 DDE domain.
    Contains 1 HTH CENPB-type DNA-binding domain.
  • 細胞内局在
    Nucleus. Chromosome. Cytoplasm. According to some authors, it is not localized to mitotic chromatin (PubMed:19244240). Recruited to trimethylated 'Lys-9' of histone H3.
  • Information by UniProt
  • 参照データベース
  • 別名
    • KIAA0461 antibody
    • MGC71543 antibody
    • Pogo transposable element with ZNF domain antibody
    • POGZ antibody
    • POGZ_HUMAN antibody
    • putative protein product of Nbla00003 antibody
    • SUHW5 antibody
    • suppressor of hairy wing homolog antibody
    • Suppressor of hairy wing homolog 5 antibody
    • Zinc finger protein 280E antibody
    • Zinc finger protein 635 antibody
    • ZNF280E antibody
    • ZNF635 antibody
    • ZNF635M antibody
    see all

画像

  • Anti-POGZ antibody (ab66652) at 5 µg/ml + Brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 155 kDa
    Observed band size : 45,>90 kDa (why is the actual band size different from the predicted?)
    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling POGZ with ab66652 at 4-8µg/ml. Arrows indicate positively labelled alveolar cells. Magnification: 400X.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analyss of human kidney tissue labelling POGZ with ab66652 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubules. Magnification: 400X.

参考文献

ab66652 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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