Anti-Pofut1 抗体 (ab74302)

製品の概要

  • 製品名Anti-Pofut1 antibody
    Pofut1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Pofut1
  • 特異性ab74302 detects endogenous levels of total Pofut1 protein.
  • アプリケーション適用あり: ELISA, WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic peptide derived from an internal region of human Pofat1.

  • ポジティブ・コントロール
    • WB: Extracts from HeLa, Jurkat and HepG2 cells. IHC-P: Human lung carcinoma tissue.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab74302 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/10000.
WB 1/500 - 1/1000. Predicted molecular weight: 44 kDa.
IHC-P 1/50 - 1/100.
ICC/IF 1/100.

ターゲット情報

  • 機能Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in EGF domains. Plays a crucial role in Notch signaling.
  • 組織特異性Highly expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • パスウェイProtein modification; protein glycosylation.
  • 配列類似性Belongs to the glycosyltransferase 68 family.
  • 細胞内局在Endoplasmic reticulum.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FUT 12 antibody
    • FUT12 antibody
    • GDP fucose protein O fucosyltransferase 1 antibody
    • GDP-fucose protein O-fucosyltransferase 1 antibody
    • O Fuc T antibody
    • o fucosyltransferase protein antibody
    • O FucT 1 antibody
    • O FucT antibody
    • O FucT1 antibody
    • O FUT antibody
    • O-FucT-1 antibody
    • OFUT 1 antibody
    • OFUT antibody
    • OFUT1 antibody
    • OFUT1_HUMAN antibody
    • Peptide O fucosyltransferase1 antibody
    • Peptide-O-fucosyltransferase 1 antibody
    • Pofut 1 antibody
    • POFUT1 antibody
    • Protein O fucosyltransferase1 antibody
    see all

Anti-Pofut1 antibody 画像

  • ab74302, at a 1/50 dilution, staining Pofut1 in paraffin embedded human lung carcinoma tissue in the absence (left image) and presence (right image) of the immunising peptide.
  • All lanes : Anti-Pofut1 antibody (ab74302) at 1/500 dilution

    Lane 1 : extracts from HeLa cells
    Lane 2 : extracts from Jurkat cells
    Lane 3 : extracts from HepG2 cells
    Lane 4 : extracts from Jurkat cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 44 kDa
    Observed band size : 44 kDa
    Additional bands at : 117 kDa,22 kDa,28 kDa,30 kDa. We are unsure as to the identity of these extra bands.

Anti-Pofut1 antibody (ab74302) 使用論文

ab74302 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (NIH3T3)
Specification NIH3T3
Fixative Paraformaldehyde
Blocking step Serum as blocking agent for 1 minute(s) · Concentration: 10% · Temperature: 24°C
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投稿 Mar 22 2013

As for ab74302,I have received information that we do not have immunogen peptide available for sale in that case.

I apologize for any inconvenience.

Please do not hesitate to contact us if you need any more advice or information in...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"