製品の概要

  • 製品名Anti-PODXL antibody
    PODXL 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PODXL
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    A region within synthetic peptide: RGGGSGNPTT TIESPKSTKS ADTTTVATST ATAKPNTTSS QNGAEDTTNS, corresponding to N terminal amino acids 109-158 of Human PODXL

  • ポジティブ・コントロール
    • HepG2 cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab62594 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 5 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1:312500.

ターゲット情報

  • 機能Involved in the regulation of both adhesion and cell morphology and cancer progression. Function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up inital epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells.
  • 組織特異性Glomerular epithelium cell (podocyte).
  • 配列類似性Belongs to the podocalyxin family.
  • ドメインBoth the O-glycan-rich domain of the extracellular domain and the C-terminus PDZ-binding motif (DTHL) in the cytoplasmic tail harbor an apical sorting signal. The cytoplasmic domain is necessary for the apical membrane targeting and renal tubulogenesis. The cytoplasmic C-terminus PDZ-binding motif (DTHL) is essential for interaction with SLC9A3R1 and for targeting SLC9A3R1 to the apical cell membrane. The extracellular domain is necessary for microvillus formation (By similarity). The large highly anionic extracellular domain allows to maintain open filtration pathways between neighboring podocyte foot processes.
  • 翻訳後修飾N- and O-linked glycosylated. Sialoglycoprotein.
  • 細胞内局在Apical cell membrane. Cell projection, lamellipodium. Cell projection, filopodium. Cell projection, ruffle. Cell projection, microvillus. Membrane raft. Membrane. In single attached epithelial cells is restricted to a preapical pole on the free plasma membrane whereas other apical and basolateral proteins are not yet polarized. Colocalizes with SLC9A3R2 at the apical plasma membrane during epithelial polarization. Colocalizes with SLC9A3R1 at the trans-Golgi network (transiently) and at the apical plasma membrane. Its association with the membrane raft is transient. Colocalizes with actin filaments, EZR and SLC9A3R1 in a punctate pattern at the apical cell surface where microvilli form. Colocalizes with EZR and SLC9A3R2 at the apical cell membrane of glomerular epithelium cells (By similarity). Forms granular, punctuated pattern, forming patches, preferentially adopting a polar distribution, located on the migrating poles of the cell or forming clusters along the terminal ends of filipodia establishing contact with the endothelial cells. Colocalizes with the submembrane actin of lamellipodia, particularly associated with ruffles. Colocalizes with vinculin at protrusions of cells. Colocalizes with ITGB1. Colocalizes with PARD3, PRKCI, EXOC5, OCLN, RAB11A and RAB8A in apical membrane initiation sites (AMIS) during the generation of apical surface and luminogenesis (By similarity).
  • Information by UniProt
  • 参照データベース
  • 製品の状態There are 2 isoforms produced by alternative splicing.
  • 別名
    • GCTM-2 antigen antibody
    • Gp2 antibody
    • Gp200 antibody
    • MGC138240 antibody
    • PC antibody
    • PCLP antibody
    • PCLP-1 antibody
    • PCLP1 antibody
    • Pcx antibody
    • Podocalyxin antibody
    • Podocalyxin like antibody
    • Podocalyxin like protein antibody
    • Podocalyxin-like protein 1 antibody
    • Podxl antibody
    • PODXL_HUMAN antibody
    see all

Anti-PODXL antibody 画像

  • Anti-PODXL antibody (ab62594) at 5 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 55 kDa
    Observed band size : 55 kDa
    Additional bands at : 34 kDa. We are unsure as to the identity of these extra bands.

Anti-PODXL antibody (ab62594) 使用論文

ab62594 has not yet been referenced specifically in any publications.

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