Anti-Podoplanin / gp36 抗体 [RTD4E10] (ab11936)

製品の概要

  • 製品名
    Anti-Podoplanin / gp36 antibody [RTD4E10]
    Podoplanin / gp36 一次抗体 製品一覧
  • 製品の詳細
    Syrian Hamster monoclonal [RTD4E10] to Podoplanin / gp36
  • 特異性
    The monoclonal antibody will detect podoplanin on the surface of mouse lymphatic endothelial cells and some epithelial cell types by immunostaining or immunohistochemistry.
  • アプリケーション
    適用あり: IHC-FoFr, IHC-Fr, IHC-P, IP, WB, Flow Cytmore details
  • 種交差性
    交差種: Mouse
    非交差種: Human
  • 免疫原

    Tissue, cells or virus corresponding to Mouse Podoplanin/ gp36. Monoclonal antibodies were produced by immunizing Syrian hamsters with a murine thymic stromal cell line.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab11936 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FoFr 1/100 - 1/250.
IHC-Fr Use at an assay dependent concentration. PubMed: 18829396
IHC-P Use a concentration of 1 - 10 µg/ml.
IP Use a concentration of 2 - 10 µg/ml.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 36 kDa.
Flow Cyt Use at an assay dependent concentration. PubMed: 19064696

ab18474 - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能
    May be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. Required for normal lung cell proliferation and alveolus formation at birth. Induces platelet aggregation. Does not have any effect on folic acid or amino acid transport. Does not function as a water channel or as a regulator of aquaporin-type water channels.
  • 組織特異性
    Highly expressed in placenta, lung, skeletal muscle and brain. Weakly expressed in brain, kidney and liver. In placenta, expressed on the apical plasma membrane of endothelium. In lung, expressed in alveolar epithelium. Up-regulated in colorectal tumors and expressed in 25% of early oral squamous cell carcinomas.
  • 配列類似性
    Belongs to the podoplanin family.
  • 翻訳後修飾
    Extensively O-glycosylated. Contains sialic acid residues. O-glycosylation is necessary for platelet aggregation activity.
    The N-terminus is blocked.
  • 細胞内局在
    Membrane. Cell projection > filopodium membrane. Cell projection > lamellipodium membrane. Cell projection > microvillus membrane. Cell projection > ruffle membrane. Localized to actin-rich microvilli and plasma membrane projections such as filopodia, lamellipodia and ruffles.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Aggrus antibody
    • Glycoprotein 36 KD antibody
    • Glycoprotein 36 antibody
    • gp 36 antibody
    • GP 38 antibody
    • GP 40 antibody
    • gp36 antibody
    • GP38 antibody
    • GP40 antibody
    • HT1A 1 antibody
    • HT1A1 antibody
    • hT1alpha 1 antibody
    • hT1alpha 2 antibody
    • hT1alpha1 antibody
    • hT1alpha2 antibody
    • Lung type I cell membrane associated glycoprotein antibody
    • Lung type I cell membrane associated glycoprotein isoform a antibody
    • Lung type I cell membrane associated glycoprotein T1A 2 antibody
    • OTS 8 antibody
    • OTS8 antibody
    • OTTHUMP00000009640 antibody
    • OTTHUMP00000044504 antibody
    • PA2.26 antibody
    • PA2.26 antigen antibody
    • Pdpn antibody
    • PDPN_HUMAN antibody
    • Podoplanin antibody
    • PSEC0003 antibody
    • PSEC0025 antibody
    • T1 alpha antibody
    • T1 ALPHA GENE antibody
    • T1-alpha antibody
    • T1A 2 antibody
    • T1A antibody
    • TI1A antibody
    • TIA 2 antibody
    • TIA2 antibody
    see all

Anti-Podoplanin / gp36 antibody [RTD4E10] 画像

  • ab11936 staining Podoplanin / gp36 in murine skin tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed in paraformaldehyde and an enzymatic antigen retrieval step was performed using Protein K. Samples were then blocked with 5% serum for 30 minutes at room temperature and then incubated with ab11936 at a 1/400 dilution for 16 hours at 4°C. The secondary used was a biotin conjugated goat anti-hamster IgG used at a 1/500 dilution.

    See Abreview

  • ab11936 staining Podoplanin / gp36 in mouse lung tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde. Samples were incubated with primary antibody (1/80 in PBS + 1% BSA) for 16 hours at 4°C. A Biotin-conjugated goat anti-hamster IgG polyclonal (1/500) was used as the secondary antibody.

    Podoplanin is revealed in green (Alexa Fluor® 488) and nucleus are counterstain in blue (DAPI). Streptavidine-Alexa Fluor® 488 was used to increase signal.

    See Abreview

  • Immunohistochemistry (PFA perfusion fixed frozen sections) analysis of mouse liver tissue sections labeling Podoplanin / gp36 with ab11936 at 1/250 dilution. Cells were fixed with formaldehyde, followed by blocking with PBS + 0.1% Triton X-100, 1% BSA, 10% goat serum for 1 hour at 25°C. A polyclonal goat anti-hamster Alexa Fluor 488 secondary antibody was used at 1/500 dilution.

    See Abreview

  • Flow Cytometry analysis of mouse lung cell effusion labeling Podoplanin / gp36 with ab11936 at 1/100 dilution.

    See Abreview

Anti-Podoplanin / gp36 antibody [RTD4E10] (ab11936) 使用論文

This product has been referenced in:
  • Gardenier JC  et al. Topical tacrolimus for the treatment of secondary lymphedema. Nat Commun 8:14345 (2017). IHC-P ; Mouse . Read more (PubMed: 28186091) »
  • Chen Y  et al. Du-Huo-Ji-Sheng-Tang Attenuates Inflammation of TNF-Tg Mice Related to Promoting Lymphatic Drainage Function. Evid Based Complement Alternat Med 2016:7067691 (2016). IHC-P ; Mouse . Read more (PubMed: 27239212) »

See all 23 Publications for this product

Product Wall

Thank you for contacting us.

Here is a link to a Histonet post which you may have already seen. The advice is generally to use clean, positively-charged slides (Superfrost Plus from Fisher is mentioned often, here and in other posts), and ve...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
IHC - Wholemount
Sample
Mouse Embryo (haert, epicardium)
Specification
haert, epicardium
Username

Abcam user community

Verified customer

投稿 Dec 14 2016

Application
Flow Cytometry
Sample
Mouse Cell (Mouse lung Effusion)
Permeabilization
No
Gating Strategy
all viable cells
Specification
Mouse lung Effusion
Preparation
Cell harvesting/tissue preparation method: established primary cell line
Sample buffer: culture medium
Username

Dr. Xiao-Ming Sun

Verified customer

投稿 Sep 14 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer ph=6
Sample
Rat Tissue sections (Lung)
Specification
Lung
Permeabilization
No
Fixative
Formaldehyde
Username

Dr. Francois Daubeuf

Verified customer

投稿 Mar 11 2015

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Lung)
Specification
Lung
Permeabilization
No
Fixative
Formaldehyde
Username

Dr. Francois Daubeuf

Verified customer

投稿 Feb 26 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer ph=6
Sample
Mouse Tissue sections (Colon)
Specification
Colon
Permeabilization
No
Fixative
Formaldehyde
Username

Dr. Francois Daubeuf

Verified customer

投稿 Feb 17 2015

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum (5%), BSA (3.75%) as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 4°C
Antigen retrieval step
None
Sample
Rat Tissue sections (Rat skin tissue)
Specification
Rat skin tissue
Permeabilization
No
Fixative
Acetone
Username

Mr. Timothy Mwangi

Verified customer

投稿 Jun 13 2014

Thank you for your enquiry and your interest in our products.

I can confirm that the clone number of ab11936 is RTD4E10 which can be found in the brackets of the target name as well as on the on-line product datasheet under the clone number. ...

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Thank you for your recent telephone enquiry.

As discussed on the telephone, far as we are aware, ab10288 Podoplanin / gp36 antibody [18H5] has never been tested with samples from mouse. All tested species cross-reactivity information is stat...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Liver)
Specification
Liver
Fixative
Formaldehyde
Antigen retrieval step
None
Blocking step
PBS + 0.1% Triton X-100, 1% BSA, 10% goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Temperature: 25°C
Username

Mr. justin kenkel

Verified customer

投稿 Jul 28 2012

1-10 of 21 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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