製品の概要

  • 製品名Anti-PNPT1 antibody
    PNPT1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PNPT1
  • アプリケーション適用あり: WB, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment, corresponding to a sequence within amino acids 1-753 of Human PNPT1.

  • ポジティブ・コントロール
    • A431 whole cell lysates. This antibody gave a positive result in IHC in the following FFPE tissue: Human testis seminoma. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: CaCo2.

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab96176 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 86 kDa.
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 関連性PNPT1 is a subunit of the exosome complex, which is involved in 3-prime-to-5-prime exoribonuclease activity for RNA processing and degradation. It contains one KH domain and one S1 motif domain.
  • 細胞内局在Mitochondrion
  • 参照データベース
  • 別名
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    • Polyribonucleotide nucleotidyltransferase 1, mitochondrial antibody
    see all

Anti-PNPT1 antibody 画像

  • ICC/IF image of ab96176 stained CaC02 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab96176 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of PNPT1 staining in Human testis seminoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab96176, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-PNPT1 antibody (ab96176) at 1/1000 dilution + Jurkat whole cell lysate at 30 µg

    Predicted band size : 86 kDa

Anti-PNPT1 antibody (ab96176) 使用論文

ab96176 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"