製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to PNPLA8
  • 特異性
    This antibody detects endogenous levels of total PNPLA8 protein.
  • アプリケーション
    適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    A synthesized peptide derived from the internal region of human PNPLA8.

  • ポジティブ・コントロール
    • Extracts from Hela cells, A549 cells and HUVEC cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab74242 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA
WB
  • 追加情報
    ELISA: 1/40000.
    WB: 1/500 - 1/1000. Detects bands of approximately 34, 48 and 88 kDa (predicted molecular weight: 88 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Calcium-independent phospholipase A2, which catalyzes the hydrolysis of the sn-2 position of glycerophospholipids, PtdSer and to a lower extent PtdCho. Cleaves membrane phospholipids.
    • 組織特異性
      Expressed in parenchymal tissues including heart, skeletal muscle, placenta, brain, liver and pancreas. Also expressed in bronchial epithelial cells and kidney. Highest expression is observed in skeletal muscle and heart.
    • 配列類似性
      Contains 1 patatin domain.
    • 細胞内局在
      Endoplasmic reticulum membrane. Golgi apparatus membrane. Cytoplasm > perinuclear region.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Calcium-independent phospholipase A2-gamma antibody
      • Intracellular membrane associated calcium independent phospholipase A2 gamma antibody
      • Intracellular membrane-associated calcium-independent phospholipase A2 gamma antibody
      • IPLA2 2 antibody
      • iPLA2 gamma antibody
      • IPLA2(GAMMA) antibody
      • iPLA2-2 antibody
      • iPLA2-gamma antibody
      • IPLA22 antibody
      • IPLA2G antibody
      • Patatin like phospholipase domain containing protein 8 antibody
      • Patatin-like phospholipase domain-containing protein 8 antibody
      • PLPL8_HUMAN antibody
      • PNPLA-gamma antibody
      • PNPLA8 antibody
      see all

    画像

    • All lanes : Anti-PNPLA8 antibody (ab74242) at 1/500 dilution

      Lane 1 : Extracts from Hela cells
      Lane 2 : Extracts from A549 cells
      Lane 3 : Extracts from HUVEC cells
      Lane 4 : Extracts from HUVEC cells plus 5µg immunizing peptide

      Lysates/proteins at 5 µg per lane.


      Predicted band size : 88 kDa
      Observed band size : 88 kDa
      Additional bands at : 34 kDa,48 kDa. We are unsure as to the identity of these extra bands.

    プロトコール

    参考文献

    ab74242 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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