製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to PNPLA3
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide corresponding to Human PNPLA3 aa 150-250 conjugated to Keyhole Limpet Haemocyanin (KLH).
    (Peptide available as ab102097)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following lysates: Human Liver Tissue; HepG2 Whole Cell Lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab81874 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 53 kDa).

ターゲット情報

  • 関連性
    PNPLA3 is a multifunctional enzyme which has both triacylglycerol lipase and acylglycerol O-acyltransferase activities. It contains one patatin domain.
  • 細胞内局在
    Membrane; Single pass type II membrane protein
  • 参照データベース
  • 別名
    • Acylglycerol O acyltransferase antibody
    • Adiponutrin antibody
    • ADPN antibody
    • C22orf20 antibody
    • Calcium independent phospholipase A2 epsilon antibody
    • DJ796I17.1 antibody
    • iPLA(2)epsilon antibody
    • IPLA2-epsilon antibody
    • Patatin like phospholipase domain containing 3 antibody
    • patatin like phospholipase domain containing protein 3 antibody
    • phospholipase A2, calcium independent, epsilon antibody
    see all

画像

  • All lanes : ab81874 at 1 µg/ml

    Lane 1 : Human liver
    Lane 2 : Human whole cell lysate HepG2

    Lysates/proteins at 10 µg per lane.

    Secondary
    ab97080 at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 53 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 120 kDa,48 kDa. We are unsure as to the identity of these extra bands.

    The PNPLA3 sequence contains two glycosylation sites (Swissprot data). The additional band close to the band of interest might be a glycosylated form of PNPLA3. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

参考文献

This product has been referenced in:
  • Liu Z  et al. Overexpression of variant PNPLA3 gene at I148M position causes malignant transformation of hepatocytes via IL-6-JAK2/STAT3 pathway in low dose free fatty acid exposure: a laboratory investigation in vitro and in vivo. Am J Transl Res 8:1319-38 (2016). Human . Read more (PubMed: 27186262) »
  • Xu F  et al. SIRT1 mediates the effect of GLP-1 receptor agonist exenatide on ameliorating hepatic steatosis. Diabetes 63:3637-46 (2014). WB ; Mouse . Read more (PubMed: 24947350) »

See all 3 Publications for this product

レビューと Q&A

Application
Western blot
Sample
Cow Tissue lysate - other (Adipose tissue)
Gel Running Conditions
Non-reduced Denaturing (4-12%)
Loading amount
25 µg
Specification
Adipose tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Sophia Erb

Verified customer

投稿 Aug 18 2016

Thank you for your interest in our antibodies. We unfortunately do not have any small samplers of any of our antibodies. I suggest trying ab81874. We have not tested it for reactivity with mouse samples, but the immunogen has 93% identity with mouse PN...

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DISCOUNT CODE: Expiration date: I am very pleased to hear you would like to accept our offer and test ab81874 inWB on bovine. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abrev...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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