製品の概要

  • 製品名
    Anti-PMP70 antibody (Biotin)
    PMP70 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PMP70 (Biotin)
  • 標識
    Biotin
  • 特異性
    ab28499 recognises Peroxisomal membrane protein 70 (PMP70).
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Chicken
  • 免疫原

    Synthetic peptide:

    NYEFKKITEDTVEFGS

    , corresponding to C terminal amino acids 644 - 659 of Rat PMP70

  • ポジティブ・コントロール
    • Mouse kidney protein extract

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab28499 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500. Predicted molecular weight: 74 kDa.

ターゲット情報

  • 機能
    Probable transporter. The nucleotide-binding fold acts as an ATP-binding subunit with ATPase activity.
  • 配列類似性
    Belongs to the ABC transporter superfamily. ABCD family. Peroxisomal fatty acyl CoA transporter (TC 3.A.1.203) subfamily.
    Contains 1 ABC transmembrane type-1 domain.
    Contains 1 ABC transporter domain.
  • 細胞内局在
    Peroxisome membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 70 kDa peroxisomal membrane protein antibody
    • ABC 43 antibody
    • ABC D3 antibody
    • ABC43 antibody
    • ABCD 3 antibody
    • Abcd3 antibody
    • ABCD3 protein antibody
    • ABCD3_HUMAN antibody
    • ATP binding cassette sub family D (ALD) member 3 antibody
    • ATP binding cassette sub family D member 3 antibody
    • ATP-binding cassette sub-family D member 3 antibody
    • CBAS5 antibody
    • dJ824O18.1 (ATP-binding cassette, sub family D (ALD), member 3 (PMP70, PXMP1)) antibody
    • Peroxisomal membrane protein 1 (70kD) antibody
    • Peroxisomal membrane protein 1 (70kD, Zellweger syndrome) antibody
    • Peroxisomal membrane protein 1 antibody
    • PMP 70 antibody
    • PMP70 antibody
    • PXMP 1 antibody
    • PXMP1 antibody
    • ZWS2 antibody
    see all

画像

  • All lanes : Anti-PMP70 antibody (Biotin) (ab28499) at 1/1000 dilution

    Lane 1 : Mouse lung tissue lysate
    Lane 2 : Mouse liver tissue lysate
    Lane 3 : HepG2 cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    HRP-conjugated anti-rabbit

    Predicted band size : 74 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescent analysis of PMP70 staining in A459 cells with ab28499.

  • Immunofluorescent analysis of PMP70 staining in NS-1 cells with ab28499.

  • Immunofluorescent analysis of PMP70 staining in HMVEC cells with ab28499.

  • Immunofluorescent analysis of PMP70 staining in p19 cells with ab28499.

  • Flow cytometry analysis of PMP70 in HepG2 cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x106 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with ab28499 at a dilution of 0.5 μg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight® 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.

  • Flow cytometry analysis of PMP70 in 293T cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x106 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with ab28499 at a dilution of 0.5 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight® 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.

プロトコール

参考文献

This product has been referenced in:
  • Falcon A  et al. FATP2 is a hepatic fatty acid transporter and peroxisomal very long-chain acyl-CoA synthetase. Am J Physiol Endocrinol Metab 299:E384-93 (2010). Purification ; Mouse . Read more (PubMed: 20530735) »

See 1 Publication for this product

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