This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »
Synthetic peptide conjugated to KLH derived from within residues 500 - 600 of Human PML+RARa Fusion. This peptide comprises the region across the PML-RAR alpha junction in the L-type fusion protein.
(Peptide available as ab46898.)
Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use
アプリケーション | Abreviews | 特記事項 |
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WB | 1/5000. |
Anti-PML+ RARα Fusion antibody (ab43152) reactivity with reduced NB4 (A and B) and K562 (B) cell lysate. After SDS-PAGE (where 100μg lysate was loaded in A and 20μg lysate in B), membranes were blocked in 5% Milk in PBS + 0.1% Tween for 1h at 25°C before incubation with ab43152 (1:5,000 dilution in 1% Milk PBS + 0.1% Tween) for 16h at 4ºC. Blots was then incubated with an anti-Rabbit HRP-conjugated secondary antibody before developing with ECL.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"