製品の概要
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製品名Anti-PML+RARA Fusion antibody
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製品の詳細Rabbit polyclonal to PML+RARA Fusion
This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »
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由来種Rabbit
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種交差性
交差が予測される動物種: Human
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 500 - 600 of Human PML+RARa Fusion. This peptide comprises the region across the PML-RAR alpha junction in the L-type fusion protein.
(Peptide available as ab46898.)
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
アプリケーション
Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/5000. |
ターゲット情報
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関連性PML protein is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This phosphoprotein localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. Retinoic acid, a metabolite of vitamin A, is necessary for normal organogenesis but acts as a teratogen at high levels during embryonic and fetal development. Retinoic acid functions through its interaction with the nuclear protein, retinoic acid receptor (RAR). RAR belongs to the steroid and thyroid hormone superfamily of nuclear receptor proteins which exert their effects by binding to specific DNA response elements, thus regulating gene expression in target cells. RAR exists as three major subtypes: alpha, beta and gamma.
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細胞内局在Nuclear and Cytoplasmic
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参照データベース
- Entrez Gene: 5371 Human
- Entrez Gene: 5914 Human
- Omim: 102578 Human
- Omim: 180240 Human
- SwissProt: P10276 Human
- SwissProt: P29590 Human
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別名
- MYL antibody
- NR1B1 antibody
- Nuclear receptor subfamily 1 group B member 1 antibody
see all
画像
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Western blot - Anti-PML+RARA Fusion antibody (ab43152)Image courtesy of Christian Marx (University of Jena, Germany)Anti-PML+ RARα Fusion antibody (ab43152) reactivity with reduced NB4 (A and B) and K562 (B) cell lysate. After SDS-PAGE (where 100μg lysate was loaded in A and 20μg lysate in B), membranes were blocked in 5% Milk in PBS + 0.1% Tween for 1h at 25°C before incubation with ab43152 (1:5,000 dilution in 1% Milk PBS + 0.1% Tween) for 16h at 4ºC. Blots was then incubated with an anti-Rabbit HRP-conjugated secondary antibody before developing with ECL.
プロトコール
参考文献
This product has been referenced in:
- Li K et al. TRIB3 Promotes APL Progression through Stabilization of the Oncoprotein PML-RARa and Inhibition of p53-Mediated Senescence. Cancer Cell 31:697-710.e7 (2017). Read more (PubMed: 28486108) »
- Li X et al. Chemosensitivity enhancement toward arsenic trioxide by inhibition of histone deacetylase in NB4 cell line. J Int Med Res 44:882-92 (2016). Read more (PubMed: 27189198) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"