製品の概要

  • 製品名Anti-PML Protein antibody [PML-97]
    PML Protein 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [PML-97] to PML Protein
  • アプリケーション適用あり: WB, IP, ICC, Indirect ELISAmore details
  • 種交差性
    交差種: Mouse, Hamster, Human
  • 免疫原

    Recombinant full-length human PML (promyelocytic leukemia) protein

  • ポジティブ・コントロール
    • 293T cells expressing recombinant PML3

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab50637 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IP
ICC
Indirect ELISA
  • 追加情報ICC: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.
    I-ELISA: Use at an assay dependent dilution.
    WB: Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 69 kDa.
    Array: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Key component of PML nuclear bodies that regulate a large number of cellular processes by facilitating post-translational modification of target proteins, promoting protein-protein contacts, or by sequestering proteins. Functions as tumor suppressor. Required for normal, caspase-dependent apoptosis in response to DNA damage, FAS, TNF, or interferons. Plays a role in transcription regulation, DNA damage response, DNA repair and chromatin organization. Plays a role in processes regulated by retinoic acid, regulation of cell division, terminal differentiation of myeloid precursor cells and differentiation of neural progenitor cells. Required for normal immunity to microbial infections. Plays a role in antiviral response. In the cytoplasm, plays a role in TGFB1-dependent processes. Regulates p53/TP53 levels by inhibiting its ubiquitination and proteasomal degradation. Regulates activation of p53/TP53 via phosphorylation at 'Ser-20'. Sequesters MDM2 in the nucleolus after DNA damage, and thereby inhibits ubiquitination and degradation of p53/TP53. Regulates translation of HIF1A by sequestering MTOR, and thereby plays a role in neoangiogenesis and tumor vascularization. Regulates RB1 phosphorylation and activity. Required for normal development of the brain cortex during embryogenesis. Can sequester herpes virus and varicella virus proteins inside PML bodies, and thereby inhibit the formation of infectious viral particles. Regulates phosphorylation of ITPR3 and plays a role in the regulation of calcium homeostasis at the endoplasmic reticulum (By similarity). Regulates transcription activity of ELF4. Inhibits specifically the activity of the tetrameric form of PKM2. Together with SATB1, involved in local chromatin-loop remodeling and gene expression regulation at the MHC-I locus. Regulates PTEN compartmentalization through the inhibition of USP7-mediated deubiquitinylation.
    • 関連疾患Note=A chromosomal aberration involving PML may be a cause of acute promyelocytic leukemia (APL). Translocation t(15;17)(q21;q21) with RARA. The PML breakpoints (type A and type B) lie on either side of an alternatively spliced exon.
    • 配列類似性Contains 2 B box-type zinc fingers.
      Contains 1 RING-type zinc finger.
    • ドメインInteracts with PKM2 via its coiled-coil domain.
      Binds arsenic via the RING-type zinc finger.
    • 翻訳後修飾Ubiquitinated; mediated by RNF4, SIAH1 or SIAH2 and leading to subsequent proteasomal degradation. 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination by RNF4 is polysumoylation-dependent.
      Undergoes 'Lys-11'-linked sumoylation. Sumoylation on all three sites is required for nuclear body formation. Sumoylation on Lys-160 is a prerequisite for sumoylation on Lys-65. The PML-RARA fusion protein requires the coiled-coil domain for sumoylation. Desumoylated by SENP2 and SENP6. Arsenic induces PML and PML-RARA oncogenic fusion proteins polysumoylation and their subsequent RNF4-dependent ubiquitination and proteasomal degradation, and is used as treatment in acute promyelocytic leukemia (APL).
      Phosphorylated in response to DNA damage, probably by ATR.
      Acetylation may promote sumoylation and enhance induction of apoptosis.
    • 細胞内局在Nucleus > nucleoplasm. Cytoplasm. Nucleus > PML body. Nucleus > nucleolus. Endoplasmic reticulum membrane. Early endosome membrane. Sumoylated forms localize to the PML nuclear bodies. The B1 box and the RING finger are also required for this nuclear localization. Isoforms lacking a nuclear localization signal are cytoplasmic. Detected in the nucleolus after DNA damage. Sequestered in the cytoplasm by interaction with rabies virus phosphoprotein.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Acure promyelocytic leukemia, inducer of antibody
      • MYL antibody
      • Pml antibody
      • PML_HUMAN antibody
      • PP8675 antibody
      • Probable transcription factor PML antibody
      • Promyelocytic leukemia antibody
      • Promyelocytic leukemia inducer of antibody
      • Promyelocytic leukemia protein antibody
      • Protein PML antibody
      • RING finger protein 71 antibody
      • RNF 71 antibody
      • RNF71 antibody
      • TRIM 19 antibody
      • Tripartite motif protein TRIM19 antibody
      • Tripartite motif-containing protein 19 antibody
      see all

    Anti-PML Protein antibody [PML-97] (ab50637) 使用論文

    This product has been referenced in:
    • Ivanauskiene K  et al. The PML-associated protein DEK regulates the balance of H3.3 loading on chromatin and is important for telomere integrity. Genome Res 24:1584-94 (2014). Human . Read more (PubMed: 25049225) »
    • Delbarre E  et al. DAXX-dependent supply of soluble (H3.3-H4) dimers to PML bodies pending deposition into chromatin. Genome Res 23:440-51 (2013). WB ; Human . Read more (PubMed: 23222847) »

    See all 4 Publications for this product

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    J'ai compris que vous aviez rencontré des difficultés avec deux anti-PML et j'aimerais donc vous rappeler que nos produits sont garantis pour fonctionner tels qu'ils sont décrits sur la fiche technique et que nous pouvons donc touj...

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    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step Heat-inactivated normal donkey serum in 0.05% PBS-T as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Antigen retrieval step None
    Sample Mouse Tissue sections (Mouse, brain tissue, whole brain sections, striatu)
    Specification Mouse, brain tissue, whole brain sections, striatu
    Permeabilization Yes - Tween-20
    Fixative Paraformaldehyde
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    投稿 Sep 16 2013

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    Thank you for your recent phone call today. I'm sorry to hear the last vial of ab50637 did not work for you and would like to confirm a new free of charge order is on its way for you. The reference is order 272291, it will be dispatched today. Than...

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