製品の概要

  • 製品名Anti-Pma1 antibody [40B7]
  • 製品の詳細
    Mouse monoclonal [40B7] to Pma1
  • 特異性Reacts with Pma1p based on the size of the band detected on western blots and the immunofluorescence localization pattern.
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Saccharomyces cerevisiae
  • 免疫原

    Raised against yeast membrane preps, screened by immunofluorescence and blotting.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • バッファーPreservative: 10mM Sodium Azide
    Constituents: Tissue Culture Supernatant
  • 精製度Tissue culture supernatant
  • 特記事項(精製)Sterile filtered.
  • ポリ/モノモノクローナル
  • クローン名40B7
  • アイソタイプIgM
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab4645 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000. Predicted molecular weight: 100 kDa. (cell lysates, ECL detection). For non-ECL western detection methods, 1/1000 - 1/5000. Also detects Pma1p-containing fusion proteins on western blots.
ICC/IF Use at an assay dependent dilution.

ターゲット情報

  • 関連性Pma1p is a plasma membrane ATPase.
  • 参照データベース
    • 別名
      • Plasma membrane ATPase 1 antibody
      • Plasma membrane H+ ATPase antibody
      • Pma 1 antibody
      • Pma1p antibody
      • Proton pump 1 antibody
      see all

    Anti-Pma1 antibody [40B7] 画像

    • For Pma1 staining cells were grown to a density of 1 A600/ml, rapidly fixed by direct addition of formaldehyde for 30 minutes, and then harvested and fixed overnight with 4.4% formaldehyde in 0.1 m potassium phosphate, pH 6.5. Before staining, fixed cells were permeabilized with 2% SDS. They were then stained with ab4645 followed by incubation with goat anti-mouse IgG conjugated to Alexa Fluor 488. Stained cells were visualized under fluorescein fluorescence optics on a Zeiss Axioplan 2 fluorescence microscope. Nomarski images (left panels) and indirect immunofluorescence (right panels) are shown for each field.

    Anti-Pma1 antibody [40B7] (ab4645) 使用論文

    This product has been referenced in:
    • Smardon AM  et al. The RAVE complex is an isoform-specific V-ATPase assembly factor in yeast. Mol Biol Cell 25:356-67 (2014). ICC/IF ; Saccharomyces cerevisiae . Read more (PubMed: 24307682) »
    • Epstein T  et al. Separation of metabolic supply and demand: aerobic glycolysis as a normal physiological response to fluctuating energetic demands in the membrane. Cancer Metab 2:7 (2014). WB . Read more (PubMed: 24982758) »

    See all 13 Publications for this product

    Product Wall

    Thank you very much for your reply.

    The anti-IgG (H&L) secondary will bind to the conserved light chains of the IgM primary. I suggest using the secondary at a higher concentration than you normally would with an IgG primary, but I'd expe...

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    Thank you very much for contacting us and letting us know about the trouble with this antibody.

    Thank you also for sending so many details about your protocol. I do have a couple of questions, just so I can further understand the situation: <...

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    Thank you for contacting Abcam.

    Under our Abpromise, we guarantee this antibody to work in western on yeast samples. As we had not tested in oh human samples, then we could not guarantee that it would work on this species.

    I would be ...

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    Thank you for your enquiry.

    Below is a selection of antibodies that are suitable for detecting the plasma membrane and nuclear envelope. They all work in western blot and I would review the datasheets to confirm which species they are tested...

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    Merci beaucoup pour votre intérêt pour nos anticorps. Nous avons trois anticorps actuellement qui pourront correspondre a vos besoin. Veuillez vérifier leur fiche technique s'il vous plaît. Je n'ai pas trouvé un anticorps testé contre pichia pa...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Saccharomyces cerevisiae Cell lysate - other (Yeast membrane preparation)
    Loading amount 5 µg
    Specification Yeast membrane preparation
    Gel Running Conditions Reduced Denaturing (7.5%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 Aug 07 2009

    Thank you for your enquiry. The isotype of this antibody is IgG3 with kappa light chains. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Thank you for your enquiry. All the information we have on species cross reactivity is specified on the datasheet, these are updated as soon as any new information is brought to our attention. As far as we are aware, cross reactivity with Canida a...

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    The Pma1p protein is very antigenic, and antibodies to it turn up frequently if yeast membrane preps are used as the immunogen. However, what the exact antigenic region is, is not currently known.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"