製品の概要
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製品名Anti-Plzf antibody [5B3]
Plzf 一次抗体 製品一覧 -
製品の詳細Mouse monoclonal [5B3] to Plzf
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由来種Mouse
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アプリケーション適用あり: ELISA, WB, ICC/IF, Flow Cytmore details
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種交差性交差種: Human
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免疫原
Purified recombinant fragment of Human Plzf expressed in E. Coli.
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ポジティブ･コントロール
- Hela cell lysate. Hela cells
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特記事項
This product was changed from ascites to supernatant. Lot no’s high than GR161367-22 are from Tissue Culture Supernatant
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
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バッファーPreservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度Protein G purified
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特記事項（精製）Purified from tissue culture supernatant.
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ポリ/モノモノクローナル
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クローン名5B3
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アイソタイプIgG1
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研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab104854 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA | 1/10000. | |
| WB | 1/500 - 1/2000. Predicted molecular weight: 74 kDa. | |
| ICC/IF | Use at an assay dependent concentration. | |
| Flow Cyt | 1/50. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能Probable transcription factor. May play a role in myeloid maturation and in the development and/or maintenance of other differentiated tissues. Probable substrate-recognition component of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
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組織特異性Within the hematopoietic system, PLZF is expressed in bone marrow, early myeloid cell lines and peripheral blood mononuclear cells. Also expressed in the ovary, and at lower levels, in the kidney and lung.
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パスウェイProtein modification; protein ubiquitination.
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関連疾患Skeletal defects, genital hypoplasia, and mental retardation
A chromosomal aberration involving ZBTB16 may be a cause of acute promyelocytic leukemia (APL). Translocation t(11;17)(q32;q21) with RARA. -
配列類似性Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 1 BTB (POZ) domain.
Contains 9 C2H2-type zinc fingers. -
細胞内局在Nucleus.
- Information by UniProt
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参照データベース
- Entrez Gene: 7704 Human
- Omim: 176797 Human
- SwissProt: Q05516 Human
- Unigene: 591945 Human
- Unigene: 682144 Human
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別名
- AI467657 antibody
- lu antibody
- PLZF antibody
see all
画像
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![Western blot - Anti-Plzf antibody [5B3] (ab104854)](http://a.abcam.com/ps/products/104/ab104854/Images/Plzf-Primary-antibodies-ab104854-1.jpg)
Western blot - Anti-Plzf antibody [5B3] (ab104854)Anti-Plzf antibody [5B3] (ab104854) at 1/500 dilution + HeLa cell lysate
Predicted band size: 74 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-Plzf antibody [5B3] (ab104854)](http://a.abcam.com/ps/products/104/ab104854/Images/Plzf-Primary-antibodies-ab104854-2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Plzf antibody [5B3] (ab104854)Immunofluorescence analysis of Hela cells using ab104854 (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. -
![Flow Cytometry - Anti-Plzf antibody [5B3] (ab104854)](http://a.abcam.com/ps/products/104/ab104854/Images/Plzf-Primary-antibodies-ab104854-3.jpg)
Flow Cytometry - Anti-Plzf antibody [5B3] (ab104854)Overlay histogram showing K562 cells stained with ab104854 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab104854, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
プロトコール
参考文献
This product has been referenced in:
- Yu-Ching Lin Z et al. Gene expression ontogeny of spermatogenesis in the marmoset uncovers primate characteristics during testicular development. Dev Biol N/A:N/A (2015). IHC-P ; Common marmoset . Read more (PubMed: 25624265) »
- McConnell MJ et al. Post transcriptional control of the epigenetic stem cell regulator PLZF by sirtuin and HDAC deacetylases. Epigenetics Chromatin 8:38 (2015). Read more (PubMed: 26405459) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"