製品の概要

  • 製品名Anti-PLVAP antibody
    PLVAP 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to PLVAP
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Fusion protein:

    AREAKLQAECSRQTQLALEEKAVLRKERDNLAKELEEKKREAEQLRMELA IRNSALDTCIKTKSQPMMPVSRPMGPVPNPQPIDPASLEEFKRKILESQR

    , corresponding to amino acids 331/430 of Human PLVAP.

  • 特記事項Produced from outbred CD1 mice


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファーConstituents: 50% Glycerol
  • 精製度Whole antiserum
  • 一次抗体 備考This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab21848 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 50 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

ターゲット情報

  • 機能Involved in the formation of stomatal and fenestral diaphragms of caveolae. May function in microvascular permeability.
  • 組織特異性Expressed in lung, kidney, heart, aorta, placenta, muscle, pituitary gland, adrenals, mammary gland, bladder, lymph node, bone marrow, trachea, digestive tract, liver and tumor-associated endothelium.
  • 細胞内局在Cell membrane. Membrane > caveola. Cytoplasm > perinuclear region. Membrane-associated protein of caveolae. Found in fenestral and stomatal diaphragms in fenestrated endothelia and transendothelial channels. Also colocalized with CAV1 in perinuclear region.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FELS antibody
    • Fenestrated endothelial linked structure protein antibody
    • Fenestrated endothelial-linked structure protein antibody
    • gp68 antibody
    • Hypothetical protein DKFZp667O074 antibody
    • Meca 32 antigen antibody
    • MECA32 antigen antibody
    • Panendothelial cell antigen antibody
    • Plasmalemma vesicle associated protein antibody
    • Plasmalemma vesicle protein 1 antibody
    • Plasmalemma vesicle-associated protein antibody
    • Plvap antibody
    • PLVAP_HUMAN antibody
    • PV 1 antibody
    • PV-1 antibody
    • PV1 antibody
    • PV1 protein antibody
    see all

Anti-PLVAP antibody 画像

  • All lanes : Anti-PLVAP antibody (ab21848) at 1/1000 dilution

    Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a negative control fusion protein with an irrelevant antigen at 20 ug
    Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the antigen fusion protein at 20 ug

    Secondary
    Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution

    Predicted band size : 50 kDa

Anti-PLVAP antibody (ab21848) 使用論文

ab21848 has not yet been referenced specifically in any publications.

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