製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to PLTP
  • アプリケーション
    適用あり: IHC-Fr, ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Recombinant full length protein (Human)

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab18990 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr 1/50.
ICC/IF Use a concentration of 1 - 10 µg/ml.
WB 1/500. Predicted molecular weight: 55 kDa.

ターゲット情報

  • 機能
    Converts HDL into larger and smaller particles. May play a key role in extracellular phospholipid transport and modulation of hdl particles.
  • 組織特異性
    Wide tissue distribution. Placenta > pancreas > lung > kidney > heart > liver > skeletal muscle > brain.
  • 配列類似性
    Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BPI fold containing family E antibody
    • BPIFE antibody
    • HDLCQ9 antibody
    • High density lipoprotein cholesterol level quantitative trait locus 9, included antibody
    • Lipid transfer protein II antibody
    • OD107 antibody
    • Phospholipid transfer protein antibody
    • Plasma phospholipid transfer protein antibody
    • Pltp antibody
    • PLTP_HUMAN antibody
    see all

画像

  • ICC/IF image of ab18990 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18990, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

参考文献

This product has been referenced in:
  • Chenau J  et al. The cell line secretome, a suitable tool for investigating proteins released in vivo by tumors: application to the study of p53-modulated proteins secreted in lung cancer cells. J Proteome Res 8:4579-91 (2009). WB ; Human . Read more (PubMed: 19639960) »
  • Desrumaux CM  et al. Phospholipid transfer protein is present in human atherosclerotic lesions and is expressed by macrophages and foam cells. J Lipid Res 44:1453-61 (2003). IHC-P ; Human . Read more (PubMed: 12730304) »

See all 2 Publications for this product

レビューと Q&A

Thank you for contacting Abcam.

The only western blot images that we have for ab18990 are those that are found in the following reference, PubMed: 19639960.

Also, we have not tested this antibody in whole mount sections and so we ca...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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