Anti-PLSCR3 抗体 (ab57554)

製品の概要

  • 製品名Anti-PLSCR3 antibody
    PLSCR3 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to PLSCR3
  • アプリケーション適用あり: WB, ICC/IF, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1-296 of Human PLSCR3

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab57554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml.
ICC/IF 1/100.
Flow Cyt 1/200. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能May mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane. May play a central role in the initiation of fibrin clot formation, in the activation of mast cells and in the recognition of apoptotic and injured cells by the reticuloendothelial system.
  • 組織特異性Expressed in heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, uterus, small intestine and peripheral blood lymphocytes. Not detected in testis, brain and liver.
  • 配列類似性Belongs to the phospholipid scramblase family.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Ca(2+)-dependent phospholipid scramblase 3 antibody
    • Phospholipid scramblase 3 antibody
    • PL scramblase 3 antibody
    • PLS3 antibody
    • PLS3_HUMAN antibody
    • Plscr3 antibody
    see all

Anti-PLSCR3 antibody 画像

  • ab57554 staining PLSCR3 in RAW264.7 cells from Mouse macrophages by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton 0.1% + 2% BSA in PBS and blocked with 2% BSA for 60 minutes at 24°C. Samples were incubated with primary antibody (1/100n PBS + 2%BSA) for 16 hours at 4°C. An  Alexa Fluor®488-conjugated Goat anti-mouse polyclonal(1/1000) was used as the secondary antibody.

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  • Western blot against tagged recombinant protein immunogen using ab57554 PLSCR3 antibody at 1ug/ml. Predicted band size of immunogen is 59 kDa
  • ab57554 at a 1/200 dilution detecting PLSCR3 in human monocytes by Flow Cytometry. The secondary used was an Alexa Fluor 488 conjugated goat anti-mouse IgG (H+L) used at a 1/500 dilution.

    See Abreview

Anti-PLSCR3 antibody (ab57554) 使用論文

ab57554 has not yet been referenced specifically in any publications.

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The image you are looking at is a western blot against a tagged full length recombinant protein. Since the tag is GST, 59 kd is the expected size.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (RAW264.7)
Specification RAW264.7
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton + 2% BSA in PBS
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 24°C
Username

Dr. Mahesh Shivananjappa

Verified customer

投稿 Apr 16 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (Monocytes from Apheresis)
Specification Monocytes from Apheresis
Fixation BD Cytofix/Cytoperm 20 min
Permeabilization Yes - BD Cytofix/Cytoperm 20 min
Gating Strategy Monocytes
Username

Dr. Mahesh Shivananjappa

Verified customer

投稿 Dec 07 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Platelets)
Loading amount 20 µg
Specification Platelets
Treatment ADP for 30 min
Gel Running Conditions Reduced Denaturing (4-20%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Mahesh Shivananjappa

Verified customer

投稿 Nov 23 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"