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Our Abpromise guarantee covers the use of ab57554 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 5 µg/ml.|
|Flow Cyt||1/200. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.|
ab57554 staining PLSCR3 in RAW264.7 cells from Mouse macrophages by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton 0.1% + 2% BSA in PBS and blocked with 2% BSA for 60 minutes at 24°C. Samples were incubated with primary antibody (1/100n PBS + 2%BSA) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-mouse polyclonal(1/1000) was used as the secondary antibody.
ab57554 has not yet been referenced specifically in any publications.