Anti-PLSCR3 抗体 (ab57554)

製品の概要

  • 製品名
  • 製品の詳細
    Mouse monoclonal to PLSCR3
  • アプリケーション
    適用あり: WB, ICC/IF, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1-296 of Human PLSCR3

  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab57554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml.
ICC/IF 1/100.
Flow Cyt 1/200. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能
    May mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane. May play a central role in the initiation of fibrin clot formation, in the activation of mast cells and in the recognition of apoptotic and injured cells by the reticuloendothelial system.
  • 組織特異性
    Expressed in heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, uterus, small intestine and peripheral blood lymphocytes. Not detected in testis, brain and liver.
  • 配列類似性
    Belongs to the phospholipid scramblase family.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Ca(2+)-dependent phospholipid scramblase 3 antibody
    • Phospholipid scramblase 3 antibody
    • PL scramblase 3 antibody
    • PLS3 antibody
    • PLS3_HUMAN antibody
    • Plscr3 antibody
    see all

Anti-PLSCR3 antibody 画像

  • ab57554 staining PLSCR3 in RAW264.7 cells from Mouse macrophages by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton 0.1% + 2% BSA in PBS and blocked with 2% BSA for 60 minutes at 24°C. Samples were incubated with primary antibody (1/100n PBS + 2%BSA) for 16 hours at 4°C. An  Alexa Fluor®488-conjugated Goat anti-mouse polyclonal(1/1000) was used as the secondary antibody.

    See Abreview

  • Western blot against tagged recombinant protein immunogen using ab57554 PLSCR3 antibody at 1ug/ml. Predicted band size of immunogen is 59 kDa
  • ab57554 at a 1/200 dilution detecting PLSCR3 in human monocytes by Flow Cytometry. The secondary used was an Alexa Fluor 488 conjugated goat anti-mouse IgG (H+L) used at a 1/500 dilution.

    See Abreview

Anti-PLSCR3 antibody (ab57554) 使用論文

ab57554 has not yet been referenced specifically in any publications.

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Thank you for contacting us.


The image you are looking at is a western blot against a tagged full length recombinant protein. Since the tag is GST, 59 kd is the expected size.

I hope this information is helpful to you. Pleas...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (RAW264.7)
Specification
RAW264.7
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton + 2% BSA in PBS
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 24°C
Username

Dr. Mahesh Shivananjappa

Verified customer

投稿 Apr 16 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (Monocytes from Apheresis)
Specification
Monocytes from Apheresis
Fixation
BD Cytofix/Cytoperm 20 min
Permeabilization
Yes - BD Cytofix/Cytoperm 20 min
Gating Strategy
Monocytes
Username

Dr. Mahesh Shivananjappa

Verified customer

投稿 Dec 07 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Platelets)
Loading amount
20 µg
Specification
Platelets
Treatment
ADP for 30 min
Gel Running Conditions
Reduced Denaturing (4-20%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Mahesh Shivananjappa

Verified customer

投稿 Nov 23 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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