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Recombinant full length protein, corresponding to amino acids 1-296 of Human PLSCR3
Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.
Our Abpromise guarantee covers the use of ab57554 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 5 µg/ml.|
|Flow Cyt||1/200. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.|
ab57554 staining PLSCR3 in RAW264.7 cells from Mouse macrophages by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton 0.1% + 2% BSA in PBS and blocked with 2% BSA for 60 minutes at 24°C. Samples were incubated with primary antibody (1/100n PBS + 2%BSA) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-mouse polyclonal(1/1000) was used as the secondary antibody.
ab57554 has not yet been referenced specifically in any publications.