Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma (left) and breast carcinoma (right) tissues labelling PLRG1 with ab86050 at 1/200 (1µg/ml). Detection: DAB and a DyLight® 594-conjugated goat anti-rabbit IgG (H+L) (1/100).
Western blot - Anti-PLRG1 antibody (ab86050)
All lanes : Anti-PLRG1 antibody (ab86050) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique
Predicted band size : 57 kDa Observed band size : 57 kDa Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.
Detection of PLRG1 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab86050 at 3 µg/mg lysate for IP and at 0.4 µg/ml for subsequent Western blot detection. Detection: Chemiluminescence with exposure time of 3 seconds.
van Maldegem F et al. CTNNBL1 facilitates the association of CWC15 with CDC5L and is required to maintain the abundance of the Prp19 spliceosomal complex. Nucleic Acids Res43:7058-69 (2015).
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