Anti-PLOD3 抗体 (ab89263)
Key features and details
- Mouse polyclonal to PLOD3
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PLOD3 antibody
PLOD3 一次抗体 製品一覧 -
製品の詳細
Mouse polyclonal to PLOD3 -
由来種
Mouse -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein within Human PLOD3. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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ポジティブ・コントロール
- HepG2 cell lysate, PLOD3 transfected 293T cell lysate and HeLa cell
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab89263の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 85 kDa.
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ICC/IF | (1) |
Use a concentration of 10 µg/ml.
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特記事項 |
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WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 85 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links. -
関連疾患
Defects in PLOD3 are the cause of lysyl hydroxylase 3 deficiency (LH3 deficiency) [MIM:612394]; also known as bone fragility with contractures arterial rupture and deafness. LH3 deficiency is a connective tissue disorder. The syndrome is characterized by congenital malformations severely affecting many tissues and organs and revealing features of several collagen disorders, most of them involving COL2A1 (type II collagen). The findings suggest that the failure of lysyl hydroxylation and hydroxylysyl carbohydrate addition, which affects many collagens, is the molecular basis of this syndrome. -
配列類似性
Contains 1 Fe2OG dioxygenase domain. -
細胞内局在
Rough endoplasmic reticulum membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 8985 Human
- Omim: 603066 Human
- SwissProt: O60568 Human
- Unigene: 153357 Human
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別名
- 2-oxoglutarate 5-dioxygenase 3 antibody
- bone protein I antibody
- BP-I antibody
see all
画像
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Anti-PLOD3 antibody (ab89263) at 5 µg/ml + HepG2 cel lysate at 50 µg
Predicted band size: 85 kDa -
All lanes : Anti-PLOD3 antibody (ab89263) at 5 µg/ml
Lane 1 : PLOD3 transfected 293T cell lysate
Lane 2 : Non-transfected lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 85 kDa -
ab89263 at 10µg/ml staining PLOD3 on HeLa cell by immunofluorescence
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (3)
ab89263 は 3 報の論文で使用されています。
- Chen Y et al. PLOD3 contributes to HER-2 therapy resistance in gastric cancer through FoxO3/Survivin pathway. Cell Death Discov 8:321 (2022). PubMed: 35835735
- Shao S et al. Lysyl Hydroxylase Inhibition by Minoxidil Blocks Collagen Deposition and Prevents Pulmonary Fibrosis via TGF-ß1/Smad3 Signaling Pathway. Med Sci Monit 24:8592-8601 (2018). PubMed: 30481795
- Lin Q et al. Analysis of colorectal cancer glyco-secretome identifies laminin β-1 (LAMB1) as a potential serological biomarker for colorectal cancer. Proteomics 15:3905-20 (2015). PubMed: 26359947