製品の概要

  • 製品名
  • 製品の詳細
    Mouse polyclonal to PLOD2
  • アプリケーション
    適用あり: WB, ICC/IFmore details
    適用なし: IHC-R
  • 種交差性
    交差種: Human
  • 免疫原

    Full length Human PLOD2 protein (NP_891988.1, 1 a.a. ~ 758 a.a)

  • ポジティブ・コントロール
    • Human kidney tissue lysate. PLOD2 transfected 293T cell lysate. HeLa cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab72939 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).
ICC/IF Use a concentration of 10 µg/ml.
  • 追加情報
    Is unsuitable for IHC-R.
  • ターゲット情報

    • 機能
      Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links.
    • 組織特異性
      Highly expressed in pancreas and muscle. Isoform 1 and isoform 2 are expressed in the majority of the examined cell types. Isoform 2 is specifically expressed in skin, lung, dura and aorta.
    • 関連疾患
      Defects in PLOD2 are the cause of Bruck syndrome type 2 (BRKS2) [MIM:609220]. Bruck syndrome, also known as osteogenesis imperfecta with congenital joint contractures, is an autosomal recessive disease characterized by generalized osteopenia, joint contractures at birth, fragile bones and short stature. It can be distinguished from osteogenesis imperfecta by the absence of hearing loss and dentinogenesis imperfecta, and by the presence of clubfoot and congenital joint limitations. The molecular defect is an aberrant cross-linking of bone collagen, due to underhydroxylation of lysine residues within the telopeptides of type I collagen, whereas the lysine residues in the triple helix are normal.
    • 配列類似性
      Contains 1 Fe2OG dioxygenase domain.
    • 細胞内局在
      Rough endoplasmic reticulum membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 2-oxoglutarate 5-dioxygenase 2 antibody
      • LH2 antibody
      • Lysine hydroxylase 2 antibody
      • Lysyl hydroxylase 2 antibody
      • OTTHUMP00000215204 antibody
      • OTTHUMP00000215205 antibody
      • OTTHUMP00000215206 antibody
      • PLOD 2 antibody
      • Plod2 antibody
      • PLOD2_HUMAN antibody
      • Procollagen lysine 2 oxoglutarate 5 dioxygenase 2 antibody
      • Procollagen lysine, 2 oxoglutarate 5 dioxygenase (lysine hydroxylase) 2 antibody
      • Procollagen-lysine antibody
      • Telopeptide lysyl hydroxylase antibody
      • TLH antibody
      see all

    画像

    • Anti-PLOD2 antibody (ab72939) at 1 µg/ml + human kidney tissue lysate at 25 µg

      Secondary
      Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

      Predicted band size : 85 kDa
      Observed band size : 85 kDa
    • All lanes : Anti-PLOD2 antibody (ab72939) at 1 µg/ml

      Lane 1 : PLOD2 transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

      Predicted band size : 85 kDa
      Observed band size : 75 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.
    • ab72939, at 10µg/ml, staining PLOD2 in Hela cells by immunofluorescence.

    参考文献

    ab72939 has not yet been referenced specifically in any publications.

    レビューと Q&A

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Resin sections)
    Sample
    Human Tissue sections (Nasal polyp, tonsil)
    Specification
    Nasal polyp, tonsil
    Username

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    投稿 Oct 12 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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