製品の概要

  • 製品名Anti-PLK4 antibody
    PLK4 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to PLK4
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Mouse, Cow, Dog
  • 免疫原

    Synthetic peptide:

    ATCIGEKIEDFKVGN

    , corresponding to N terminal amino acids 2-16 of Human PLK4.

  • ポジティブ・コントロール
    • Human colon lysate
  • 特記事項GenBank Accession Number - NP_055079

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab2642 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 109 kDa).Can be blocked with Human PLK4 peptide (ab22924).

ターゲット情報

  • 機能Serine/threonine-protein kinase that plays a central role in centriole duplication. Able to trigger procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment of centriole biogenesis proteins such as SASS6, CENPJ/CPAP, CP110, CEP135 and gamma-tubulin. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. Its central role in centriole replication suggests a posible role in tumorigenesis, centrosome aberrations being frequently observed in tumors. Also involved in trophoblast differentiation by phosphorylating HAND1, leading to disrupt the interaction between HAND1 and MDFIC and activate HAND1. Phosphorylates CDC25C and CHEK2/CHK2.
  • 配列類似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.
    Contains 1 POLO box domain.
    Contains 1 protein kinase domain.
  • 翻訳後修飾Ubiquitinated; leading to its degradation by the proteasome.
    Tyrosine-phosphorylated by TEC.
  • 細胞内局在Cytoplasm > cytoskeleton > centrosome > centriole. Nucleus > nucleolus. Cleavage furrow. Associates with centrioles throughout the cell cycle. According to PubMed:16244668, it is not present at cleavage furrows.
  • Information by UniProt
  • 参照データベース
  • 別名
    • PLK 4 antibody
    • PLK-4 antibody
    • PLK4 antibody
    • PLK4_HUMAN antibody
    • Polo like Kinase 4 antibody
    • Polo-like kinase 4 antibody
    • Protein serine/threonine kinase antibody
    • SAK antibody
    • Serine/Threonine Kinase 18 antibody
    • Serine/threonine kinase antibody
    • Serine/threonine protein kinase 18 antibody
    • Serine/threonine protein kinase PLK4 antibody
    • Serine/threonine protein kinase Sak antibody
    • Serine/threonine-protein kinase 18 antibody
    • Serine/threonine-protein kinase PLK4 antibody
    • Serine/threonine-protein kinase SAK antibody
    • Snk Akin Kinase antibody
    • STK 18 antibody
    • STK18 antibody
    see all

Anti-PLK4 antibody 画像

  • Anti-PLK4 antibody (ab2642) at 2 µg/ml + human colon lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 109 kDa
    Observed band size : 110 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 hour

Anti-PLK4 antibody (ab2642) 使用論文

This product has been referenced in:
  • Czub B  et al. TOPORS, a Dual E3 Ubiquitin and Sumo1 Ligase, Interacts with 26 S Protease Regulatory Subunit 4, Encoded by the PSMC1 Gene. PLoS One 11:e0148678 (2016). Read more (PubMed: 26872363) »
  • Rosario CO  et al. A novel role for Plk4 in regulating cell spreading and motility. Oncogene N/A:N/A (2014). Read more (PubMed: 25174401) »

See all 4 Publications for this product

Product Wall

I have organised a new vial to be sent to you, you should get a confirmation e-mail for this "order" soon. Please could you let me know how you get on with this batch? I would very much appreciate your feedback.

The antibody in stock is a new batch and therefore has not been tested by other customers. I have arranged a credit note to be issued to you. This antibody sells well and the reported problems are therefore few. I wish you all the best with your r...

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I'm terribly sorry you are having problems with ab2642. We have a new batch in stock which I could send to you or I can arrange a refund or credit note. Please let me know which option you would prefer and accept my apologies for this problem, I loo...

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Thank you for your e-mail. I am very sorry you are not getting the right band, especially with your positive control. I will arrange for a new replacement vial to be sent to you. I understand you bought the antibody from Gene Research. Can you please ...

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Thank you for the information. I have now a clearer idea of your problem and would like to help by suggesting some modifications to your protocol. I think the problem might be a degradation of your protein before it is recognised by the antibody as ...

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Thank you for your e-mail. Please find enclosed the protocol. Tissue Lysis: Tissue chunks were weighed and cut into approx 1mm cubes using a razor blade. The tissue was transferred to a handheld homogenizer and 3 ml of ice-cold RIPA buffer was ad...

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Thank you for your enquiry and for forwarding your detailed protocol. This antibody has been tested for Western blot application using Human Placenta Lysate. We would strongly suggest running a positive control along with the samples. Also you need...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"