PLK1 overexpression 293T lysate (whole cell) (ab94104)

製品の概要

  • 製品名PLK1 overexpression 293T lysate (whole cell)
  • 製品の詳細
    PLK1 overexpression lysate
  • 特記事項ab94104 is a 293T cell transfected lysate in which Human PLK1 has been transiently over-expressed using a pCMV-PLK1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed about how the transfected lysate was prepared view preparation notes
  • アプリケーション適用あり: WBmore details

法規制情報

製品の特性

  • 背景Function: Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis. Required for recovery after DNA damage checkpoint and entry into mitosis. Required for kinetochore localization of BUB1B. Phosphorylates SGOL1. Required for spindle pole localization of isoform 3 of SGOL1 and plays a role in regulating its centriole cohesion function. Phosphorylates BORA, and thereby promotes the degradation of BORA. Contributes to the regulation of AURKA function. Regulates TP53 stability through phosphorylation of TOPORS. Tissue specificity: Placenta and colon. Similarity: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily. Contains 2 POLO box domains. Contains 1 protein kinase domain. Developmental stage: Accumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase. PTM: Catalytic activity is enhanced by phosphorylation of Thr-210. Phosphorylation at Thr-210 is first detected on centrosomes in the G2 phase of the cell cycle, peaks in prometaphase and gradually disappears from centrosomes during anaphase. Autophosphorylation and phosphorylation of Ser-137 may not be significant for the activation of PLK1 during mitosis, but may enhance catalytic activity during recovery after DNA damage checkpoint. Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) in anaphase and following DNA damage, leading to its degradation by the proteasome. Ubiquitination is mediated via its interaction with FZR1/CDH1. Ubiquitination and subsequent degradation prevents entry into mitosis and is essential to maintain an efficient G2 DNA damage checkpoint.

アプリケーション

Our Abpromise guarantee covers the use of ab94104 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent dilution.

PLK1 overexpression 293T lysate (whole cell) 画像

  • ab94104 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-PLK1 antibody (ab89600) at 1/500 dilution

    Lane 1 : PLK1 overexpression 293T lysate (whole cell) (ab94104)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

PLK1 overexpression 293T lysate (whole cell) (ab94104) 使用論文

ab94104 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"