Anti-PLK1 抗体 [AZ27] (ab12210)

製品の概要

  • 製品名Anti-PLK1 antibody [AZ27]
    PLK1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [AZ27] to PLK1
  • 特異性The cross-reactivity of this antibody regards the various isoforms of PLK is not known.
  • アプリケーション適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Mouse, Human, Xenopus laevis
  • 免疫原

    Recombinant full length protein (Xenopus laevis).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab12210 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent dilution.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 68 kDa).

ターゲット情報

  • 機能Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis. Required for recovery after DNA damage checkpoint and entry into mitosis. Required for kinetochore localization of BUB1B. Phosphorylates SGOL1. Required for spindle pole localization of isoform 3 of SGOL1 and plays a role in regulating its centriole cohesion function. Phosphorylates BORA, and thereby promotes the degradation of BORA. Contributes to the regulation of AURKA function. Regulates TP53 stability through phosphorylation of TOPORS.
  • 組織特異性Placenta and colon.
  • 配列類似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.
    Contains 2 POLO box domains.
    Contains 1 protein kinase domain.
  • 発生段階Accumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase.
  • 翻訳後修飾Catalytic activity is enhanced by phosphorylation of Thr-210. Phosphorylation at Thr-210 is first detected on centrosomes in the G2 phase of the cell cycle, peaks in prometaphase and gradually disappears from centrosomes during anaphase.
    Autophosphorylation and phosphorylation of Ser-137 may not be significant for the activation of PLK1 during mitosis, but may enhance catalytic activity during recovery after DNA damage checkpoint.
    Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) in anaphase and following DNA damage, leading to its degradation by the proteasome. Ubiquitination is mediated via its interaction with FZR1/CDH1. Ubiquitination and subsequent degradation prevents entry into mitosis and is essential to maintain an efficient G2 DNA damage checkpoint.
  • 細胞内局在Nucleus. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > centrosome. During early stages of mitosis, the phosphorylated form is detected on centrosomes and kinetochores. Localizes to the outer kinetochore. Presence of SGOL1 and interaction with the phosphorylated form of BUB1 is required for the kinetochore localization.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cell cycle regulated protein kinase antibody
    • PLK 1 antibody
    • PLK antibody
    • PLK-1 antibody
    • plk1 antibody
    • PLK1_HUMAN antibody
    • Polo like kinase 1 antibody
    • Polo-like kinase 1 antibody
    • Serine/threonine protein kinase 13 antibody
    • Serine/threonine protein kinase PLK1 antibody
    • Serine/threonine-protein kinase 13 antibody
    • Serine/threonine-protein kinase PLK1 antibody
    • STPK 13 antibody
    • STPK13 antibody
    see all

Anti-PLK1 antibody [AZ27] 画像



  • Predicted band size : 68 kDa

    This image is courtesy of Julian Gannon, CRUK

    Western blot using ab12210.

    Lane 1: HeLa extract
    Lane 2: mouse Myeloid cell extract
    Lane 3: Mitotic CSF Xenopus egg extract
    Lane 4: Interphase Xenopus egg extract

    Molecular weight markers 120, 98, 68, 57, 43, 32, 18, 12 kD. Note that the phosphorylated form of Polo kinase from the Xenopus mitotic extract migrates slower than the unphosphorylated form present in the interphase extract.

    Western blot using ab12210.

    Lane 1: HeLa extract

    Lane 2: mouse Myeloid cell extract

    Lane 3: Mitotic CSF Xenopus egg extract

    Lane 4: Interphase Xenopus egg extract M

    olecular weight markers 120, 98, 68, 57, 43, 32, 18, 12 kD.

    Note that the phosphorylated form of Polo kinase from the Xenopus mitotic extract migrates slower than the unphosphorylated form present in the interphase extract.

Anti-PLK1 antibody [AZ27] (ab12210) 使用論文

ab12210 has not yet been referenced specifically in any publications.

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Thank you for your enquiry and for your patience. The originator of ab12210 has provided me with the following information: "Interesting observation but it's not uncommon for homologous proteins with similar mwts derived from different species to migra...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"