製品の概要

  • 製品名Anti-PLK1 antibody
    PLK1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PLK1
  • 特異性PLK1 Antibody detects endogenous levels of total PLK1 protein.
  • アプリケーション適用あり: ELISA, WB, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide corresponding to Human PLK1. The antiserum was produced against synthesized peptide derived from human PLK1.
    Database link: P53350

  • ポジティブ・コントロール
    • Extracts from LOVO cells treated with PMA (125ng/ml, 30mins).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab61118 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/20000.
WB 1/500 - 1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
ICC/IF 1/100 - 1/500.

ターゲット情報

  • 機能Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis. Required for recovery after DNA damage checkpoint and entry into mitosis. Required for kinetochore localization of BUB1B. Phosphorylates SGOL1. Required for spindle pole localization of isoform 3 of SGOL1 and plays a role in regulating its centriole cohesion function. Phosphorylates BORA, and thereby promotes the degradation of BORA. Contributes to the regulation of AURKA function. Regulates TP53 stability through phosphorylation of TOPORS.
  • 組織特異性Placenta and colon.
  • 配列類似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.
    Contains 2 POLO box domains.
    Contains 1 protein kinase domain.
  • 発生段階Accumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase.
  • 翻訳後修飾Catalytic activity is enhanced by phosphorylation of Thr-210. Phosphorylation at Thr-210 is first detected on centrosomes in the G2 phase of the cell cycle, peaks in prometaphase and gradually disappears from centrosomes during anaphase.
    Autophosphorylation and phosphorylation of Ser-137 may not be significant for the activation of PLK1 during mitosis, but may enhance catalytic activity during recovery after DNA damage checkpoint.
    Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) in anaphase and following DNA damage, leading to its degradation by the proteasome. Ubiquitination is mediated via its interaction with FZR1/CDH1. Ubiquitination and subsequent degradation prevents entry into mitosis and is essential to maintain an efficient G2 DNA damage checkpoint.
  • 細胞内局在Nucleus. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > centrosome. During early stages of mitosis, the phosphorylated form is detected on centrosomes and kinetochores. Localizes to the outer kinetochore. Presence of SGOL1 and interaction with the phosphorylated form of BUB1 is required for the kinetochore localization.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cell cycle regulated protein kinase antibody
    • PLK 1 antibody
    • PLK antibody
    • PLK-1 antibody
    • plk1 antibody
    • PLK1_HUMAN antibody
    • Polo like kinase 1 antibody
    • Polo-like kinase 1 antibody
    • Serine/threonine protein kinase 13 antibody
    • Serine/threonine protein kinase PLK1 antibody
    • Serine/threonine-protein kinase 13 antibody
    • Serine/threonine-protein kinase PLK1 antibody
    • STPK 13 antibody
    • STPK13 antibody
    see all

Anti-PLK1 antibody 画像

  • Immunofluorescent analysis of A549 cells labeling PLK1 with ab61118 at 1/100 dilution. The picture on the right is blocked with the synthesized peptide.

  • All lanes : Anti-PLK1 antibody (ab61118) at 1/500 dilution

    Lane 1 : Extracts from LOVO cells treated with PMA (125ng/ml, 30mins)
    Lane 2 : Extracts from LOVO cells treated with PMA (125ng/ml, 30mins) with immunising PLK1 peptide


    Predicted band size : 68 kDa
    Observed band size : 68 kDa

    Secondary antibody anti-rabbit HRP (ab6721)

Anti-PLK1 antibody (ab61118) 使用論文

ab61118 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing (10% separating gel)
Sample Mouse Tissue lysate - whole (Hippocampal homogenate)
Specification Hippocampal homogenate
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Feb 12 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"