製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to PLGF
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Cow, Pig
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human PLGF.

    (Peptide available as ab95428.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Human Placenta Tissue Lysate, and JEG-3, SW480 and RAW 264.7 whole cell lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab83906 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 26 kDa).

ターゲット情報

  • 機能
    Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner.
  • 組織特異性
    While the three isoforms are present in most placental tissues, PlGF-2 is specific to early (8 week) placenta and only PlGF-1 is found in the colon and mammary carcinomas.
  • 配列類似性
    Belongs to the PDGF/VEGF growth factor family.
  • ドメイン
    Isoform PlGF-2 contains a basic insert which acts as a cell retention signal.
  • 翻訳後修飾
    N-glycosylated.
  • 細胞内局在
    Secreted. The three isoforms are secreted but PlGF-2 appears to remain cell attached unless released by heparin.
  • Information by UniProt
  • 参照データベース
  • 別名
    • D12S1900 antibody
    • Pgf antibody
    • PGFL antibody
    • PIGF antibody
    • Placenta growth factor antibody
    • Placental growth factor antibody
    • Placental growth factor, vascular endothelial growth factor related protein antibody
    • PlGF 2 antibody
    • PlGF antibody
    • PLGF_HUMAN antibody
    • PlGF2 antibody
    • SHGC 10760 antibody
    see all

画像

  • All lanes : Anti-PLGF antibody (ab83906) at 1 µg/ml

    Lane 1 : Human placenta tissue lysate - total protein (ab29745)
    Lane 2 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
    Lane 3 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
    Lane 4 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 26 kDa
    Observed band size: 55 kDa (why is the actual band size different from the predicted?)


    Exposure time: 4 minutes


    Ab83906 detects a band of approximately 55-kDa. While this differs to its predicted molecular weight of 26-kDa, this migration has been observed in the literature (PMID: 10403932).

プロトコール

参考文献

ab83906 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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