Rabbit polyclonal to Plexin D1
Rat, Dog, Human
Synthetic peptide corresponding to Human Plexin D1 aa 1800-1900 conjugated to keyhole limpet haemocyanin.
Database link: Q9Y4D7
(Peptide available as
- This antibody gave a positive signal in Mouse brain tissue lysate.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Detects a band of approximately 230 kDa (predicted molecular weight: 212 kDa).
Detected at low levels in heart, placenta, lung, skeletal muscle, kidney, thymus and liver. Detected at very low levels in brain, colon, spleen, small intestine and peripheral blood leukocytes.
Belongs to the plexin family.
Contains 3 IPT/TIG domains.
Contains 1 Sema domain.
Information by UniProt
- KIAA0620 antibody
- MGC75353 antibody
- PLEX D1 antibody
Western blot - Anti-Plexin D1 antibody (ab93234)
Anti-Plexin D1 antibody (ab93234) at 1 µg/ml + Brain (Mouse) Tissue Lysate at 10 µgSecondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080
) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.Predicted band size:
212 kDaObserved band size:
230 kDa (why is the actual band size different from the predicted?
)Additional bands at:
71 kDa, 85 kDa. We are unsure as to the identity of these extra bands.Exposure time:
Plexin D1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
has not yet been referenced specifically in any publications.
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