製品の概要

  • 製品名Anti-Plexin A3 antibody
    Plexin A3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Plexin A3
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性

    交差が予測される動物種: Mouse, Human
  • 免疫原

    Synthetic peptide derived from within residues 1100 - 1200 of Mouse Plexin A3.

    (Peptide available as ab41563.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in P0 mouse brain and mouse hippocampus tissue lysates

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab41564 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 207 kDa (predicted molecular weight: 207 kDa).
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 関連性Plexin A3 is a coreceptor for the class 3 secreted semaphorins. This family of semaphorins binds to a complex composed of a neuropilin and a plexin. The plexin type modulates the affinity of the complex for specific semaphorins and its cytoplasmic domain is required for the activation of down-stream signaling events in the cytoplasm. Plexin A3 is a putative receptor involved in the development of neural and epithelial tissues.
  • 細胞内局在Membrane; single-pass type I membrane protein.
  • 参照データベース
  • 別名
    • HSSEXGENE antibody
    • Plexin 4 antibody
    • PLXN4 antibody
    • PLXNA3 antibody
    • Semaphorin receptor SEX antibody
    • SEX antibody
    • Transmembrane protein sex antibody
    • XAP 6 antibody
    see all

Anti-Plexin A3 antibody 画像

  • All lanes : Anti-Plexin A3 antibody (ab41564) at 1 µg/ml

    Lane 1 : P0 Mouse Brain Mouse Tissue Lysate
    Lane 2 : Hippocampus (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 207 kDa
    Observed band size : 207 kDa
    Additional bands at : 32 kDa,50 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

Anti-Plexin A3 antibody (ab41564) 使用論文

This product has been referenced in:
  • Demyanenko GP  et al. Neural cell adhesion molecule NrCAM regulates Semaphorin 3F-induced dendritic spine remodeling. J Neurosci 34:11274-87 (2014). IHC-FoFr ; Mouse . Read more (PubMed: 25143608) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (Cancer cells)
Loading amount 22 µg
Specification Cancer cells
Gel Running Conditions Reduced Denaturing (10)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Mr. Manoj Kumar Valluru

Verified customer

投稿 Jul 23 2010

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (E 14 Mouse Embryo)
Specification E 14 Mouse Embryo
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH 6
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Mr. Manoj Kumar Valluru

Verified customer

投稿 Nov 03 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"