Anti-Plexin A1 抗体 [EPR3449] (ab92346)


  • 製品名Anti-Plexin A1 antibody [EPR3449]
    Plexin A1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR3449] to Plexin A1
  • 特異性ab92346 is expected to cross react with Plexin A3 and A4 due to 100% immunogen homology with Plexin A3 and 87.5 % with Plexin A4,however not tested experimentally.
  • アプリケーション適用あり: WB, ICC, Flow Cytmore details
    適用なし: IHC-P or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human Plexin A1.

  • ポジティブ・コントロール
    • KS62, HUVEC and HepG2 cell lysates; fetal brain tissue lysate
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.



Our Abpromise guarantee covers the use of ab92346 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt
  • 追加情報Flow Cyt: 1/50.
    ICC: 1/100.
    WB: 1/1000 - 1/2000. Predicted molecular weight: 211 kDa.

    Is unsuitable for IHC-P or IP.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性Function: Coreceptor for SEMA3A, SEMA3C, SEMA3F and SEMA6D. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin. The plexin modulates the affinity of the complex for specific semaphorins, and its cytoplasmic domain is required for the activation of down-stream signaling events in the cytoplasm. Tissue specificity: Detected in fetal brain, lung, liver and kidney. Similarity: Belongs to the plexin family. Contains 4 IPT/TIG domains. Contains 1 Sema domain.
    • 細胞内局在Cell Membrane
    • 参照データベース
    • 別名
      • NOV antibody
      • NOV/plexin A1 protein antibody
      • NOVP antibody
      • Plexin-A1 antibody
      • Plexin1 antibody
      • PlexinA1 antibody
      • PLXA1_HUMAN antibody
      • PLXN1 antibody
      • Plxna1 antibody
      • PLXNA1 protein antibody
      • Semaphorin receptor NOV antibody
      see all

    Anti-Plexin A1 antibody [EPR3449] 画像

    • All lanes : Anti-Plexin A1 antibody [EPR3449] (ab92346) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : HUVEC cell lysate
      Lane 4 : HepG2 cell lysate
      Lane 5 : Fetal brain tissue lysate

      Lysates/proteins at 10 µg per lane.

      HRP labelled goat anti-rabbit antibody at 1/2000 dilution

      Predicted band size : 211 kDa

    Anti-Plexin A1 antibody [EPR3449] (ab92346) 使用論文

    ab92346 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Cell lysate - whole cell (Spleen)
    Loading amount 20 µg
    Specification Spleen
    Gel Running Conditions Reduced Denaturing (gel 7,5%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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    投稿 Jan 16 2012

    Ab92346 recognizes the extracellular domain of Plexin A1. Permeabilization is still required, even though the immunogen is extracellular.