製品の概要

  • 製品名Anti-PLEKHG2 antibody
  • 製品の詳細
    Mouse polyclonal to PLEKHG2
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length protein (Human), PLEKHG2 (AAH13426.1, 896 a.a).

  • ポジティブ・コントロール
    • PLEKHG2 transfected 293T cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab68756 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 130 kDa.

ターゲット情報

  • 機能May be a transforming oncogene with exchange activity for CDC42 (By similarity). May be a guanine-nucleotide exchange factor (GEF) for RAC1 and CDC42. Activated by the binding to subunits beta and gamma of the heterotrimeric guanine nucleotide-binding protein (G protein).
  • 配列類似性Contains 1 DH (DBL-homology) domain.
    Contains 1 PH domain.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 1110070J07Rik antibody
    • AA673237 antibody
    • CLG antibody
    • FLJ00018 antibody
    • FLJ22458 antibody
    • PH domain-containing family G member 2 antibody
    • PKHG2_HUMAN antibody
    • Pleckstrin homology domain containing family G member 2 antibody
    • Pleckstrin homology domain-containing family G member 2 antibody
    • Plekhg2 antibody
    • ZFYVE18 antibody
    see all

Anti-PLEKHG2 antibody 画像

  • All lanes : Anti-PLEKHG2 antibody (ab68756) at 1/500 dilution

    Lane 1 : PLEKHG2 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Observed band size : 130 kDa (why is the actual band size different from the predicted?)

Anti-PLEKHG2 antibody (ab68756) 使用論文

ab68756 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Skin Fibroblasts)
Loading amount 25 µg
Specification Skin Fibroblasts
Gel Running Conditions Reduced Denaturing (12.5)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Mar 18 2013

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Skin Fibroblasts)
Specification Skin Fibroblasts
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% triton
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

投稿 Dec 13 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"