Rabbit polyclonal to PLEKHA4
Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
Synthetic peptide within residues: TEPDSPSPVL QGEESSERES LPESLELSSP RSPETDWGRP PGGDKDLASP , corresponding to internal sequence amino acids 504-553 of Human PLEKHA4 (NP_065955)
- Transfected 293T cell lysate.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Immunogen affinity purified
ab84727 is purified by a peptide affinity chromatography method.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Binds specifically to phosphatidylinositol-3-phosphate (PtdIns3P), but not to other phosphoinositides.
Highly expressed in melanoma. Detected at low levels in heart, skeletal muscle, kidney, liver and small intestine.
Contains 1 PH domain.
Information by UniProt
- 2410005C22Rik antibody
- C88169 antibody
- MGC72599 antibody
Western blot - PLEKHA4 antibody (ab84727)
Anti-PLEKHA4 antibody (ab84727) at 1 µg/ml (in 5% skim milk / PBS buffer) + Transfected 293T cell lysate at 10 µg
anti-Rabbit IgG HRP at 1/50000 dilution
Predicted band size : 85 kDa
Observed band size : 85 kDa
Additional bands at : 32 kDa,50 kDa. We are unsure as to the identity of these extra bands.
has not yet been referenced specifically in any publications.
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