Anti-PLEKHA3 抗体 [2D1] (ab117849)

製品の概要

  • 製品名Anti-PLEKHA3 antibody [2D1]
    PLEKHA3 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [2D1] to PLEKHA3
  • アプリケーション適用あり: WB, Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length recombinant Human PLEKHA3 (NP_061964) produced in HEK293T cells.

  • ポジティブ・コントロール
    • HEK293T transfected lysate HeLa or Jurkat cells HEK293T transfected cells

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーpH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 1% BSA, 50% Glycerol, 48% PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Purified from mouse ascite fluid by affinity chromatography
  • ポリ/モノモノクローナル
  • クローン名2D1
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab117849 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000. Predicted molecular weight: 34 kDa.
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100.

ターゲット情報

  • 機能Binds specifically to phosphatidylinositol-4-phosphate (PtdIns4P), but not to other phosphoinositides.
  • 組織特異性Widely expressed.
  • 配列類似性Contains 1 PH domain.
  • 細胞内局在Cytoplasm. Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FAPP 1 antibody
    • FAPP-1 antibody
    • FAPP1 antibody
    • FLJ20067 antibody
    • PH domain-containing family A member 3 antibody
    • Phosphatidylinositol-four-phosphate adapter protein 1 antibody
    • Phosphoinositol 4 phosphate adaptor protein 1 antibody
    • Phosphoinositol 4-phosphate adapter protein 1 antibody
    • PKHA3_HUMAN antibody
    • Pleckstrin homology domain containing family A (phosphoinositide binding specific) member 3 antibody
    • Pleckstrin homology domain containing family A member 3 antibody
    • Pleckstrin homology domain-containing family A member 3 antibody
    • PLEKHA 3 antibody
    • Plekha3 antibody
    see all

Anti-PLEKHA3 antibody [2D1] 画像

  • All lanes : Anti-PLEKHA3 antibody [2D1] (ab117849) at 1/2000 dilution

    Lane 1 : HEK293T lysate transfected with pCMV6-ENTRY control cDNA for 48 hrs
    Lane 2 : HEK293T lysate transfected with pCMV6-ENTRY PLEKHA3 cDNA for 48 hrs

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 34 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC200386)
  • Immunofluorescent staining of HeLa cells using ab117849 at a 1/100 dilution.
  • Flow cytometry image showing ab117849 at a 1/100 dilution, staining HEK293T cells transfected with PLEKHA3 (Red), and an empty vector control plasmid (Blue).
  • Flow cytometry image staining HeLa cells, using ab117849 at a 1/100 dilution (Red), and a nonspecific negative control antibody (Blue).
  • Flow cytometry image staining Jurkat cells, using ab117849 at a 1/100 dilution (Red), and a nonspecific negative control antibody (Blue).

Anti-PLEKHA3 antibody [2D1] (ab117849) 使用論文

ab117849 has not yet been referenced specifically in any publications.

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Below please find the IF protocol recommended with this antibody:

Fixation permeabilization

Coat the coverslips with 0.1mg/ml poly-L-lysine solution at room temperature for 2hrs, dry, and then w...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"