Anti-Pleckstrin 抗体 (ab115514)
Key features and details
- Goat polyclonal to Pleckstrin
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Pleckstrin antibody
Pleckstrin 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to Pleckstrin -
由来種
Goat -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Dog -
免疫原
Synthetic peptide:
EWIKAIQMASRTGK
with a Cysteine residue linker, corresponding to C terminal amino acids 337-350 of Human Pleckstrin (NP_002655.2). -
ポジティブ・コントロール
- Human blood vessel (platelets) and spleen tissue; Human PBMC lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab115514の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
Use a concentration of 0.05 - 1 µg/ml. Predicted molecular weight: 40 kDa.
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IHC-P |
Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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WB
Use a concentration of 0.05 - 1 µg/ml. Predicted molecular weight: 40 kDa. |
IHC-P
Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Major protein kinase C substrate of platelets. -
配列類似性
Contains 1 DEP domain.
Contains 2 PH domains. - Information by UniProt
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参照データベース
- Entrez Gene: 442956 Dog
- Entrez Gene: 5341 Human
- Entrez Gene: 56193 Mouse
- Entrez Gene: 364206 Rat
- Omim: 173570 Human
- SwissProt: Q6Q308 Dog
- SwissProt: P08567 Human
- SwissProt: Q9JHK5 Mouse
see all -
別名
- FLJ27168 antibody
- OTTHUMP00000159962 antibody
- p47 antibody
see all
画像
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ab115514 at 3.75ug/ml staining Pleckstrin in Formalin-fixed, Paraffin-embedded Human vessel (platelets) tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
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ab115514 at 3.75ug/ml staining Pleckstrin in Formalin-fixed, Paraffin-embedded Human Spleen tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
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Anti-Pleckstrin antibody (ab115514) at 0.05 µg/ml + Human PBMC lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 40 kDa
Primary incubation: 1 hour
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (1)
ab115514 は 1 報の論文で使用されています。
- Lezirovitz K et al. A rare genomic duplication in 2p14 underlies autosomal dominant hearing loss DFNA58. Hum Mol Genet 29:1520-1536 (2020). PubMed: 32337552