製品の概要

  • 製品名Anti-PLD3 antibody
    PLD3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PLD3
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    A synthetic peptide corresponding to a region of Human PLD3

  • ポジティブ・コントロール
    • Fetal heart lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab76433 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1:62500.

ターゲット情報

Anti-PLD3 antibody 画像



  • Predicted band size : 55 kDa
    Western blot analysis of Human adult placenta tissue lysate labeling PLD3 with ab76433 at 1.0µg/ml.
  • Anti-PLD3 antibody (ab76433) at 1 µg/ml + fetal heart lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 55 kDa
    Observed band size : 55 kDa
    Gel concentration 12%

Anti-PLD3 antibody (ab76433) 使用論文

ab76433 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Zebrafish Tissue lysate - whole (total larvae, 4 days post fertilization)
Specification total larvae, 4 days post fertilization
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Dr. Evgenia Salta

Verified customer

投稿 Oct 27 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"