製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to PLCZ1
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Recombinant fragment, corresponding to amino acids 322-572 of Human PLCZ1 (BC125067).

  • ポジティブ・コントロール
    • HeLa cell, Human fetal liver and fetal kidney lysates, Human fetal testis tissue

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab124446 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 70 kDa.
IHC-P 1/100 - 1/500.

ターゲット情報

  • 機能
    The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. In vitro, hydrolyzes PtdIns(4,5)P2 in a Ca(2+)-dependent manner. Triggers intracellular Ca(2+) oscillations in oocytes solely during M phase and is involved in inducing oocyte activation and initiating embryonic development up to the blastocyst stage. Is therefore a strong candidate for the egg-activating soluble sperm factor that is transferred from the sperm into the egg cytoplasm following gamete membrane fusion. May exert an inhibitory effect on phospholipase-C-coupled processes that depend on calcium ions and protein kinase C, including CFTR trafficking and function.
  • 組織特異性
    Expressed specifically in testis. Weakly expressed in pancreatic-duct cells. Up-regulated in pancreatic-duct cells from patients with cystic fibrosis.
  • 配列類似性
    Contains 1 C2 domain.
    Contains 1 EF-hand domain.
    Contains 1 PI-PLC X-box domain.
    Contains 1 PI-PLC Y-box domain.
  • ドメイン
    The EF-hand and C2 domains are essential for triggering Ca(2+) oscillating activity and the regulation of PLCZ1 enzyme activity.
    The X-Y linker region between PI-PLC X-box and Y-box domains may be a target for proteolysis and may play an important regulatory role during fertilization.
  • 細胞内局在
    Nucleus. Cytoplasm > perinuclear region. Exhibits alternative cytoplasmic/nuclear localization during development. Translocates from the pronucleus into cytoplasm upon nuclear envelope breakdown for mitosis and localizes again to the pronucleus at interphase following meiosis and mitosis.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 1 phosphatidylinositol 4,5 bisphosphate phosphodiesterase zeta 1 antibody
    • 1-phosphatidylinositol-4 antibody
    • 5-bisphosphate phosphodiesterase zeta-1 antibody
    • MGC149685 antibody
    • NYD SP27 antibody
    • Phosphoinositide phospholipase C zeta 1 antibody
    • Phosphoinositide phospholipase C-zeta-1 antibody
    • Phospholipase C zeta 1 antibody
    • Phospholipase C-zeta-1 antibody
    • PI phospholipase C zeta 1 antibody
    • PLC zeta 1 antibody
    • PLC-zeta-1 antibody
    • PLCZ1 antibody
    • PLCZ1_HUMAN antibody
    • PLCzeta antibody
    • Testis development protein NYD SP27 antibody
    • Testis development related NYD SP27 antibody
    • Testis-development protein NYD-SP27 antibody
    see all

画像

  • ab124446 at 1/100 dilution staining PLCZ1 in Human fetal testis tissue by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.

参考文献

This product has been referenced in:
  • Nakasuji T  et al. Complementary Critical Functions of Zfy1 and Zfy2 in Mouse Spermatogenesis and Reproduction. PLoS Genet 13:e1006578 (2017). WB . Read more (PubMed: 28114340) »

See 1 Publication for this product

レビューと Q&A

There are currently no Customer reviews or Questions for ab124446.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録