製品の概要

  • 製品名Anti-PLCG 2 (phospho Y759) antibody [EP1406Y]
    PLCG 2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1406Y] to PLCG 2 (phospho Y759)
  • 特異性This antibody only detects PLCG 2 phosphorylated on Tyrosine 759.
  • アプリケーション適用あり: WB, IP, ICC/IFmore details
    適用なし: Flow Cyt
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic phospho peptide corresponding to residues surrounding Tyr759 of human PLCG 2.

  • ポジティブ・コントロール
    • Raji cell lysate.
  • 特記事項

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab51020 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/50000 - 1/100000. Detects a band of approximately 150 kDa (predicted molecular weight: 148 kDa).
IP 1/50.
ICC/IF 1/50 - 1/100.
  • 追加情報Is unsuitable for Flow Cyt.
  • ターゲット情報

    • 機能The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling.
    • 配列類似性Contains 1 C2 domain.
      Contains 1 PH domain.
      Contains 1 PI-PLC X-box domain.
      Contains 1 PI-PLC Y-box domain.
      Contains 2 SH2 domains.
      Contains 1 SH3 domain.
    • 翻訳後修飾Phosphorylated on tyrosine residues; upon ligand-induced activation of a variety of growth factor receptors and immune system receptors. Increases phospholipase activity.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 2 antibody
      • 1-phosphatidylinositol-4 antibody
      • 5-bisphosphate phosphodiesterase gamma-2 antibody
      • EC 3.1.4.11 antibody
      • Phosphoinositide phospholipase C antibody
      • Phosphoinositide phospholipase C-gamma-2 antibody
      • Phospholipase C gamma 2 antibody
      • Phospholipase C, gamma 2 (phosphatidylinositol specific) antibody
      • Phospholipase C-gamma-2 antibody
      • Phospholipase C-IV antibody
      • PLC 2 antibody
      • PLC gamma 2 antibody
      • PLC IV antibody
      • PLC-gamma-2 antibody
      • PLC-IV antibody
      • Plcg2 antibody
      • PLCG2_HUMAN antibody
      see all

    Anti-PLCG 2 (phospho Y759) antibody [EP1406Y] 画像

    • All lanes : Anti-PLCG 2 (phospho Y759) antibody [EP1406Y] (ab51020) at 1/100000 dilution

      Lane 1 : Raji cell lysate, untreated
      Lane 2 : Raji cell lysate, treated with pervanadate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP labeled at 1/2000 dilution

      Predicted band size : 148 kDa
      Observed band size : 150 kDa (why is the actual band size different from the predicted?)

    Anti-PLCG 2 (phospho Y759) antibody [EP1406Y] (ab51020) 使用論文

    This product has been referenced in:
    • Mohammad DK  et al. Dual phosphorylation of Btk by Akt/protein kinase b provides docking for 14-3-3?, regulates shuttling, and attenuates both tonic and induced signaling in B cells. Mol Cell Biol 33:3214-26 (2013). Read more (PubMed: 23754751) »

    See 1 Publication for this product

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    There are currently no Abreviews or Questions for ab51020.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"