Anti-PLCG 2 (phospho Y753) 抗体 (ab75659)

製品の概要

  • 製品名
    Anti-PLCG 2 (phospho Y753) antibody
    PLCG 2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PLCG 2 (phospho Y753)
  • 特異性
    ab75659 detects endogenous levels of PLCG 2 only when phosphorylated at tyrosine 753.
  • アプリケーション
    適用あり: IP, ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic phosphopeptide derived from human PLCG 2 around the phosphorylation site of tyrosine 753 (S-L-YP-D-V).

  • ポジティブ・コントロール
    • Extract from A431 cells treated with EGF (200ng/ml, 5min).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab75659 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use a concentration of 5 µg/ml.
ICC/IF 1/100 - 1/200.
WB 1/500 - 1/1000. Detects a band of approximately 148 kDa (predicted molecular weight: 148 kDa).

ターゲット情報

  • 機能
    The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling.
  • 配列類似性
    Contains 1 C2 domain.
    Contains 1 PH domain.
    Contains 1 PI-PLC X-box domain.
    Contains 1 PI-PLC Y-box domain.
    Contains 2 SH2 domains.
    Contains 1 SH3 domain.
  • 翻訳後修飾
    Phosphorylated on tyrosine residues; upon ligand-induced activation of a variety of growth factor receptors and immune system receptors. Increases phospholipase activity.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 2 antibody
    • 1-phosphatidylinositol-4 antibody
    • 5-bisphosphate phosphodiesterase gamma-2 antibody
    • EC 3.1.4.11 antibody
    • Phosphoinositide phospholipase C antibody
    • Phosphoinositide phospholipase C-gamma-2 antibody
    • Phospholipase C gamma 2 antibody
    • Phospholipase C, gamma 2 (phosphatidylinositol specific) antibody
    • Phospholipase C-gamma-2 antibody
    • Phospholipase C-IV antibody
    • PLC 2 antibody
    • PLC gamma 2 antibody
    • PLC IV antibody
    • PLC-gamma-2 antibody
    • PLC-IV antibody
    • Plcg2 antibody
    • PLCG2_HUMAN antibody
    see all

画像

  • PLCG 2 was immunoprecipitated using 0.5mg A431 whole cell extract, 5µg of Rabbit polyclonal to PLCG 2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, A431 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab75659.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 148kDa; PLCG 2

  • All lanes : Anti-PLCG 2 (phospho Y753) antibody (ab75659) at 1/500 dilution

    Lane 1 : A431 cell extracts, untreated
    Lane 2 : A431 cell extracts treated with EGF (200ng/ml, 5min)


    Predicted band size : 148 kDa
    Observed band size : 148 kDa
  • ab75659 at 1/100 dilution staining PLCG 2 (phospho Y753) in human Hela cells by ICC/IF.

参考文献

ab75659 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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