Anti-PLCG 2 (phospho Y1217) 抗体 [EP1404Y] (ab52610)
Key features and details
- Rabbit monoclonal [EP1404Y] to PLCG 2 (phospho Y1217)
- Suitable for: WB, Dot blot
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y]
PLCG 2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP1404Y] to PLCG 2 (phospho Y1217) -
由来種
Rabbit -
特異性
ab52610 only detects PLCG2 phosphorylated on Tyrosine 1217. -
アプリケーション
適用あり: WB, Dot blotmore details
適用なし: Flow Cyt,ICC/IF or IHC-P -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Ramos and Raji cell lysates treated with pervanadate.
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特記事項
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP1404Y -
アイソタイプ
IgG -
研究分野
関連製品
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab52610の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/50000. Detects a band of approximately 150 kDa (predicted molecular weight: 150 kDa).
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Dot blot |
1/1000.
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特記事項 |
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WB
1/50000. Detects a band of approximately 150 kDa (predicted molecular weight: 150 kDa). |
Dot blot
1/1000. |
ターゲット情報
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機能
The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling. -
配列類似性
Contains 1 C2 domain.
Contains 1 PH domain.
Contains 1 PI-PLC X-box domain.
Contains 1 PI-PLC Y-box domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain. -
翻訳後修飾
Phosphorylated on tyrosine residues; upon ligand-induced activation of a variety of growth factor receptors and immune system receptors. Increases phospholipase activity. - Information by UniProt
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参照データベース
- Entrez Gene: 5336 Human
- Omim: 600220 Human
- SwissProt: P16885 Human
- Unigene: 413111 Human
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別名
- 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 2 antibody
- 1-phosphatidylinositol-4 antibody
- 5-bisphosphate phosphodiesterase gamma-2 antibody
see all
画像
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All lanes : Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (ab52610) at 1/1000 dilution
Lane 1 : Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate
Lane 2 : Ramos treated with 1mM pervanadate for 30 min whole cell lysate
Lane 3 : Ramos treated with 1mM pervanadate for 30 min whole cell lysate. Then the membrane was incubated with alkaline phosphatase.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 150 kDa
Observed band size: 150 kDaBlocking and dilution buffer: 5% NFDM/TBST.
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Dot blot analysis of PLCG 2 (phospho Y1217) phospho peptide (Lane 1) and PLCG 2 phospho peptide (Lane 2) labelling PLCG 2 (phospho Y1217) with ab52610 at a dilution of 1/1000. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/20,000 dilution. Blocking buffer: 5% NFDM/TBST. Dilution buffer: 5% NFDM /TBST.
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All lanes : Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (ab52610) at 1/50000 dilution
Lane 1 : Raji cell lysate (untreated)
Lane 2 : Raji cell lysate treated with pervanadate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 150 kDa
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab52610 は論文での使用が確認できていません。