Anti-PLCG 2 (phospho Y1217) 抗体 [EP1404Y] (ab52610)


  • 製品名
    Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y]
    PLCG 2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1404Y] to PLCG 2 (phospho Y1217)
  • 特異性
    ab52610 only detects PLCG2 phosphorylated on Tyrosine 1217.
  • アプリケーション
    適用あり: WB, IP, IHC-Pmore details
    適用なし: Flow Cyt or ICC/IF
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human PLCG 2 aa 1200 to the C-terminus (phospho Y1217).

  • ポジティブ・コントロール
    • Raji cell lysate; human lymph node tissue.
  • 特記事項

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.



Our Abpromise guarantee covers the use of ab52610 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/50000. Detects a band of approximately 150 kDa (predicted molecular weight: 150 kDa).
IP 1/50.
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • 追加情報
    Is unsuitable for Flow Cyt or ICC/IF.
  • ターゲット情報

    • 機能
      The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling.
    • 配列類似性
      Contains 1 C2 domain.
      Contains 1 PH domain.
      Contains 1 PI-PLC X-box domain.
      Contains 1 PI-PLC Y-box domain.
      Contains 2 SH2 domains.
      Contains 1 SH3 domain.
    • 翻訳後修飾
      Phosphorylated on tyrosine residues; upon ligand-induced activation of a variety of growth factor receptors and immune system receptors. Increases phospholipase activity.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 2 antibody
      • 1-phosphatidylinositol-4 antibody
      • 5-bisphosphate phosphodiesterase gamma-2 antibody
      • EC antibody
      • Phosphoinositide phospholipase C antibody
      • Phosphoinositide phospholipase C-gamma-2 antibody
      • Phospholipase C gamma 2 antibody
      • Phospholipase C, gamma 2 (phosphatidylinositol specific) antibody
      • Phospholipase C-gamma-2 antibody
      • Phospholipase C-IV antibody
      • PLC 2 antibody
      • PLC gamma 2 antibody
      • PLC IV antibody
      • PLC-gamma-2 antibody
      • PLC-IV antibody
      • Plcg2 antibody
      • PLCG2_HUMAN antibody
      see all

    Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] 画像

    • ab52610 staining PLCG 2 (phospho Y1217) in human stomach tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and permeabilized; antigen retrieval was by heat mediation in Tris EDTA buffer, pH 9.0. Samples were incubated with primary antibody (1/50) for 30 minutes at 20°C. An undiluted HRP-conjugated mouse anti-rabbit IgG polyclonal was used as the secondary antibody.

      See Abreview

    • Immunohistochemical analysis of paraffin-embedded human lymph node tissue using ab52610 at a dilution of 1/50.
    • All lanes : Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (ab52610) at 1/50000 dilution

      Lane 1 : Raji cell lysate with no pervanadate
      Lane 2 : Raji cell lysate with pervanadate

      Lysates/proteins at 10 µg per lane.

      HRP-labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 150 kDa

    Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (ab52610) 使用論文

    ab52610 has not yet been referenced specifically in any publications.

    Product Wall

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: TRIS EDTA Buffer pH 9,0
    Human Tissue sections (Stomach)
    Yes - Wash Buffer from Dako with Tween

    Mr. Rudolf Jung

    Verified customer

    投稿 Mar 30 2015

    Thank you for your enquiry.

    Below please find the protocol used to test this antibody in IHC-P. Please note that citrate buffer pH 6 was used as the retreival buffer.

    I hope this is helpful. Please contact me again if you have any f...

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    Thank you for your enquiry regarding ab52610.

    The phosphorylation of PLCG 2 at Tyrosine 1217 needs to be induced to be able to detect this specific modification. As the WB image on the datasheet indicates Raji cell lysate without stimulation ...

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