Anti-PLCG 2 (phospho Y1197) 抗体 [EP1405Y] (ab75970)

製品の概要

  • 製品名Anti-PLCG 2 (phospho Y1197) antibody [EP1405Y]
    PLCG 2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1405Y] to PLCG 2 (phospho Y1197)
  • 特異性ab75970 detects PLCG 2 phosphorylated on tyrosine 1197.
  • アプリケーション適用あり: WB, IP, ICCmore details
    適用なし: Flow Cyt or IHC-P
  • 種交差性
    交差種: Human
  • 免疫原

    A phospho specific peptide corresponding to residues surrounding tyrosine 1197 of human PLCG 2.

  • ポジティブ・コントロール
    • Raji cell lysates treated with pervanadate.
  • 特記事項

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab75970 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/20000. Detects a band of approximately 150 kDa (predicted molecular weight: 148 kDa).
IP 1/40.
ICC 1/100 - 1/250.
  • 追加情報Is unsuitable for Flow Cyt or IHC-P.
  • ターゲット情報

    • 機能The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling.
    • 配列類似性Contains 1 C2 domain.
      Contains 1 PH domain.
      Contains 1 PI-PLC X-box domain.
      Contains 1 PI-PLC Y-box domain.
      Contains 2 SH2 domains.
      Contains 1 SH3 domain.
    • 翻訳後修飾Phosphorylated on tyrosine residues; upon ligand-induced activation of a variety of growth factor receptors and immune system receptors. Increases phospholipase activity.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 2 antibody
      • 1-phosphatidylinositol-4 antibody
      • 5-bisphosphate phosphodiesterase gamma-2 antibody
      • EC 3.1.4.11 antibody
      • Phosphoinositide phospholipase C antibody
      • Phosphoinositide phospholipase C-gamma-2 antibody
      • Phospholipase C gamma 2 antibody
      • Phospholipase C, gamma 2 (phosphatidylinositol specific) antibody
      • Phospholipase C-gamma-2 antibody
      • Phospholipase C-IV antibody
      • PLC 2 antibody
      • PLC gamma 2 antibody
      • PLC IV antibody
      • PLC-gamma-2 antibody
      • PLC-IV antibody
      • Plcg2 antibody
      • PLCG2_HUMAN antibody
      see all

    Anti-PLCG 2 (phospho Y1197) antibody [EP1405Y] 画像

    • All lanes : Anti-PLCG 2 (phospho Y1197) antibody [EP1405Y] (ab75970) at 1/20000 dilution

      Lane 1 : Raji cell lysates un-treated
      Lane 2 : Raji cell lysates treated with pervanadate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 148 kDa
      Observed band size : 150 kDa (why is the actual band size different from the predicted?)

    Anti-PLCG 2 (phospho Y1197) antibody [EP1405Y] (ab75970) 使用論文

    ab75970 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab75970.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"