Anti-PLCG 2 抗体 [EPR1403] (ab109267)

製品の概要

  • 製品名Anti-PLCG 2 antibody [EPR1403]
    PLCG 2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR1403] to PLCG 2
  • アプリケーション適用あり: WB, IHC-P, ICC/IFmore details
    適用なし: Flow Cyt or IP
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human PLCG 2.

  • ポジティブ・コントロール
    • Ramos and Raji cell lysates, Human kidney tissue
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Alternative versions available:

    Anti-PLCG 2 antibody (HRP) [EPR1403] (ab201242)

    Anti-PLCG 2 antibody (Alexa Fluor® 647) [EPR1403] (ab200848)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109267 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 148 kDa).
IHC-P 1/100 - 1/250. Perform antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • 追加情報Is unsuitable for Flow Cyt or IP.
  • ターゲット情報

    • 機能The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling.
    • 配列類似性Contains 1 C2 domain.
      Contains 1 PH domain.
      Contains 1 PI-PLC X-box domain.
      Contains 1 PI-PLC Y-box domain.
      Contains 2 SH2 domains.
      Contains 1 SH3 domain.
    • 翻訳後修飾Phosphorylated on tyrosine residues; upon ligand-induced activation of a variety of growth factor receptors and immune system receptors. Increases phospholipase activity.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 2 antibody
      • 1-phosphatidylinositol-4 antibody
      • 5-bisphosphate phosphodiesterase gamma-2 antibody
      • EC 3.1.4.11 antibody
      • Phosphoinositide phospholipase C antibody
      • Phosphoinositide phospholipase C-gamma-2 antibody
      • Phospholipase C gamma 2 antibody
      • Phospholipase C, gamma 2 (phosphatidylinositol specific) antibody
      • Phospholipase C-gamma-2 antibody
      • Phospholipase C-IV antibody
      • PLC 2 antibody
      • PLC gamma 2 antibody
      • PLC IV antibody
      • PLC-gamma-2 antibody
      • PLC-IV antibody
      • Plcg2 antibody
      • PLCG2_HUMAN antibody
      see all

    Anti-PLCG 2 antibody [EPR1403] 画像

    • All lanes : Anti-PLCG 2 antibody [EPR1403] (ab109267) at 1/1000 dilution

      Lane 1 : Ramos cell lysate
      Lane 2 : Raji cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 148 kDa
      Observed band size : 150 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue using ab109267 at a dilution of 1/100.

    Anti-PLCG 2 antibody [EPR1403] (ab109267) 使用論文

    ab109267 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TRIS EDTA Buffer pH 9,0
    Sample Human Tissue sections (Stomach)
    Specification Stomach
    Permeabilization Yes - Wash Buffer from Dako with Tween
    Fixative Paraformaldehyde
    Username

    Mr. Rudolf Jung

    Verified customer

    投稿 Mar 30 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"