The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 148 kDa).
1/100 - 1/250. Perform antigen retrieval before commencing with IHC staining protocol.
1/100 - 1/250.
Is unsuitable for Flow Cyt or IP.
The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling.