製品の概要

  • 製品名Anti-Plastin L antibody [EPR4278]
    Plastin L 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4278] to Plastin L
  • アプリケーション適用あり: WB, IP, IHC-P, ICC, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human Plastin L.

  • ポジティブ・コントロール
    • THP-1, Jurkat, and K562 cell lysates, Human tonsil tissue
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab109129 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000 - 1/50000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/500 - 1/1000.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Actin-binding protein. Plays a role in the activation of T-cells in response to costimulation through TCR/CD3 and CD2 or CD28. Modulates the cell surface expression of IL2RA/CD25 and CD69.
  • 組織特異性Detected in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia, in spleen and other lymph node-containing organs. Expressed in peripheral blood T lymphocytes, neutrophils, monocytes, B lymphocytes, and myeloid cells.
  • 配列類似性Contains 2 actin-binding domains.
    Contains 4 CH (calponin-homology) domains.
    Contains 2 EF-hand domains.
  • 翻訳後修飾Phosphorylated on a serine residue in response to costimulation through TCR/CD3 and CD2 or CD28. Serine phosphorylation promotes association with the actin cytoskeleton and targeting to peripheral cell projections.
    The N-terminus is blocked.
  • 細胞内局在Cytoplasm > cytoskeleton. Cell junction. Cell projection. Cell projection > ruffle membrane. Relocalizes to the immunological synapse between peripheral blood T lymphocytes and antibody-presenting cells in response to costimulation through TCR/CD3 and CD2 or CD28. Associated with the actin cytoskeleton at membrane ruffles (By similarity). Relocalizes to actin-rich cell projections upon serine phosphorylation.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CP64 antibody
    • L plastin antibody
    • L-plastin antibody
    • Larval cuticle protein 1 antibody
    • LC64P antibody
    • LCP-1 antibody
    • LCP1 antibody
    • LPL antibody
    • Lplastin antibody
    • Lymphocyte cytosolic protein 1 antibody
    • Plastin 2 antibody
    • Plastin-2 antibody
    • PLS2 antibody
    • PLSL_HUMAN antibody
    see all

Anti-Plastin L antibody [EPR4278] 画像

  • Immunohistochemical staining of paraffin-embedded Human tonsil tissue using ab109129 at a dilution of 1/250.
  • All lanes : Anti-Plastin L antibody [EPR4278] (ab109129) at 1/10000 dilution

    Lane 1 : THP-1 cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : K562 cell lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 70 kDa

Anti-Plastin L antibody [EPR4278] (ab109129) 使用論文

ab109129 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab109129.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"