The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 40 kDa.
Use a concentration of 0.5 µg/ml.
Malaria caused by Plasmodium falciparum remains the major life-threatening parasitic infection in the world. Plasmodium aldolase is an enzyme of the parasite glycolytic pathway expressed by the blood stages of Plasmodium falciparum as well as the non-falciparum malaria parasites. Monoclonal antibodies against Plasmodium aldolase are pan-specific in their reaction and have been used in an immunochromatographic test that targets the pan malarial antigen to rapidly detect malaria cases. CATALYTIC ACTIVITY: D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.
Gopal NS & Raychaudhuri R Detection of Plasmodium Aldolase Using a Smartphone and Microfluidic Enzyme Linked Immunosorbent Assay. Malar Res Treat2017:9062514 (2017).
Read more (PubMed: 29057138) »
Chen PB et al. Plasmodium falciparum PfSET7: enzymatic characterization and cellular localization of a novel protein methyltransferase in sporozoite, liver and erythrocytic stage parasites. Sci Rep6:21802 (2016).
Read more (PubMed: 26902486) »