Biotin Anti-Plasminogen 抗体 (ab7335)
Key features and details
- Biotin Goat polyclonal to Plasminogen
- Suitable for: Flow Cyt, ELISA, WB, ICC/IF
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG
製品の概要
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製品名
Biotin Anti-Plasminogen antibody
Plasminogen 一次抗体 製品一覧 -
製品の詳細
Biotin Goat polyclonal to Plasminogen -
由来種
Goat -
標識
Biotin -
アプリケーション
適用あり: Flow Cyt, ELISA, WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Full length native protein (purified) corresponding to Human Plasminogen. Human plasma
Database link: P00747 -
特記事項
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per goat IgG molecule.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Tripotassium orthophosphate -
Concentration information loading...
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精製度
IgG fraction -
特記事項(精製)
IgG fraction antibody purified from monospecific antiserum by a multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab7335の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt | (1) |
Use at an assay dependent concentration.
ab37376 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody. |
ELISA |
Use at an assay dependent concentration.
As well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. |
|
WB |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration. PubMed: 19182206
|
特記事項 |
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Flow Cyt
Use at an assay dependent concentration. ab37376 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody. |
ELISA
Use at an assay dependent concentration. As well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. |
WB
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. PubMed: 19182206 |
ターゲット情報
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機能
Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells.
Angiostatin is an angiogenesis inhibitor that blocks neovascularization and growth of experimental primary and metastatic tumors in vivo. -
組織特異性
Present in plasma and many other extracellular fluids. It is synthesized in the liver. -
関連疾患
Defects in PLG are a cause of susceptibility to thrombosis (THR) [MIM:188050]. It is a multifactorial disorder of hemostasis characterized by abnormal platelet aggregation in response to various agents and recurrent thrombi formation.
Defects in PLG are the cause of plasminogen deficiency (PLGD) [MIM:217090]. PLGD is characterized by decreased serum plasminogen activity. Two forms of the disorder are distinguished: type 1 deficiency is additionally characterized by decreased plasminogen antigen levels and clinical symptoms, whereas type 2 deficiency, also known as dysplasminogenemia, is characterized by normal, or slightly reduced antigen levels, and absence of clinical manifestations. Plasminogen deficiency type 1 results in markedly impaired extracellular fibrinolysis and chronic mucosal pseudomembranous lesions due to subepithelial fibrin deposition and inflammation. The most common clinical manifestation of type 1 deficiency is ligneous conjunctivitis in which pseudomembranes formation on the palpebral surfaces of the eye progresses to white, yellow-white, or red thick masses with a wood-like consistency that replace the normal mucosa. -
配列類似性
Belongs to the peptidase S1 family. Plasminogen subfamily.
Contains 5 kringle domains.
Contains 1 PAN domain.
Contains 1 peptidase S1 domain. -
ドメイン
Kringle domains mediate interaction with CSPG4. -
翻訳後修飾
N-linked glycan contains N-acetyllactosamine and sialic acid. O-linked glycans consist of Gal-GalNAc disaccharide modified with up to 2 sialic acid residues (microheterogeneity).
In the presence of the inhibitor, the activation involves only cleavage after Arg-580, yielding two chains held together by two disulfide bonds. In the absence of the inhibitor, the activation involves additionally the removal of the activation peptide. -
細胞内局在
Secreted. Locates to the cell surface where it is proteolytically cleaved to produce the active plasmin. Interaction with HRG tethers it to the cell surface. - Information by UniProt
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参照データベース
- Entrez Gene: 5340 Human
- Omim: 173350 Human
- SwissProt: P00747 Human
- Unigene: 143436 Human
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製品の状態
Cleaved into the following 5 chains: 1.Plasmin heavy chain A2.Activation peptide3.Angiostatin4.Plasmin heavy chain A, short form5. Plasmin light chain B -
別名
- Plasmin antibody
- Plasmin heavy chain A antibody
- Plasmin light chain B antibody
see all
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (1)
ab7335 は 1 報の論文で使用されています。
- Wygrecka M et al. Enolase-1 promotes plasminogen-mediated recruitment of monocytes to the acutely inflamed lung. Blood 113:5588-98 (2009). WB, ICC/IF, IHC-Fr ; Human . PubMed: 19182206