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Our Abpromise guarantee covers the use of ab10178 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|AP||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration. Antigen retrieval is not essential but may optimise staining.|
|Flow Cyt||Use 2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|Other||Use at an assay dependent concentration.|
ab10178 staining human brain tissue sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in EDTA prior to blocking in 10% serum for 20 hours at 37°C. The primary antibody was diluted 1/200 and incubated with the sample for 18 hours at 37°C. A biotinylated horse anti-mouse IgG antibody, diluted 1/500, was used as the secondary.