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Full length native prekallikrein protein (purified) (Human)
Our Abpromise guarantee covers the use of ab1006 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ELISA||Use at an assay dependent concentration.|
|Inhibition Assay||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration.
Dilute in PBS or medium which is identical to that used in the assay system. This antibody inhibits activation of prekallikrein by approximately 60-80%.
ab1006 staining Plasma Kallikrein 1B by Western Blot.
Lanes 1-6: Human Vitreous (4ug/lane)
Lane 7: PK
Lane 8: PK + Factor XII + HK
An HRP conjugated Goat anti-mouse antibody was used as the secondary.
The antibody was used to block the conversion of prekallikrein to kallikrein in vivo and in vitro (PMID: 17259996). The antibody also reacts with rat retina and vitreous.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"