製品の概要
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製品名Anti-PLAGL2 antibody
PLAGL2 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to PLAGL2
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アプリケーション適用あり: ICC/IF, WB, IHC-Pmore details
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種交差性交差種: Human
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免疫原
Recombinant fragment corresponding to amino acids 236-344 of Human PLAGL2
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ポジティブ･コントロール
- Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells); Human lymph node tissue.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab121239 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
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| ICC/IF | Use a concentration of 1 - 4 µg/ml. Recommend PFA Fixation and Triton X-100 treatment |
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| WB | 1/250 - 1/500. | |
| IHC-P | 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. pH 6.0 |
ターゲット情報
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機能Shows weak transcriptional activatory activity.
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配列類似性Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 6 C2H2-type zinc fingers. -
細胞内局在Nucleus.
- Information by UniProt
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参照データベース
- Entrez Gene: 5326 Human
- Omim: 604866 Human
- SwissProt: Q9UPG8 Human
- Unigene: 154104 Human
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別名
- C2H2 type zinc finger protein antibody
- KIAA0198 antibody
- PLAGL2 antibody
see all
Anti-PLAGL2 antibody 画像
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Immunocytochemistry/ Immunofluorescence - Anti-PLAGL2 antibody (ab121239)Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121239 1-4 µg/ml. Cells treated with PFA/Triton X-100. -
Western blot - Anti-PLAGL2 antibody (ab121239)All lanes : Anti-PLAGL2 antibody (ab121239) at 1/250 dilution
Lane 1 : Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2 : Negative control (vector only transfected HEK293T lysate)
Lane 3 : Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLAGL2 antibody (ab121239)Immunohistochemical staining of Paraffin embedded Human lymph node tissue, using 1/35 ab121239, shows strong cytoplasmic positivity in reaction center cells.
プロトコール
Anti-PLAGL2 antibody (ab121239) 使用論文
This product has been referenced in:
- Wang YP et al. Pleomorphic adenoma gene like-2 induces epithelial-mesenchymal transition via Wnt/ß-catenin signaling pathway in human colorectal adenocarcinoma. Oncol Rep 37:1961-1970 (2017). WB, IHC-P ; Human . Read more (PubMed: 28259923) »
- Guo J et al. Overexpression of Pleomorphic Adenoma Gene-Like 2 Is a Novel Poor Prognostic Marker of Prostate Cancer. PLoS One 11:e0158667 (2016). Read more (PubMed: 27537362) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"