製品の概要

  • 製品名
    Anti-PLAGL2 antibody
    PLAGL2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PLAGL2
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment corresponding to amino acids 236-344 of Human PLAGL2

  • ポジティブ･コントロール
    • Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells); Human lymph node tissue.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab121239 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment
WB 1/250 - 1/500.
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. pH 6.0

ターゲット情報

  • 機能
    Shows weak transcriptional activatory activity.
  • 配列類似性
    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 6 C2H2-type zinc fingers.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
    • 別名
      • C2H2 type zinc finger protein antibody
      • KIAA0198 antibody
      • PLAGL2 antibody
      • PLAL2_HUMAN antibody
      • Pleiomorphic adenoma gene-like 2 antibody
      • Pleiomorphic adenoma-like protein 2 antibody
      • Zinc finger protein PLAGL2 antibody
      • ZNF900 antibody
      see all

    画像

    • Immunocytochemistry/ Immunofluorescence - Anti-PLAGL2 antibody (ab121239)
      Immunocytochemistry/ Immunofluorescence - Anti-PLAGL2 antibody (ab121239)
      Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121239 1-4 µg/ml. Cells treated with PFA/Triton X-100.
    • Western blot - Anti-PLAGL2 antibody (ab121239)
      Western blot - Anti-PLAGL2 antibody (ab121239)
      All lanes : Anti-PLAGL2 antibody (ab121239) at 1/250 dilution

      Lane 1 : Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10

      Lane 2 : Negative control (vector only transfected HEK293T lysate)

      Lane 3 : Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLAGL2 antibody (ab121239)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLAGL2 antibody (ab121239)
      Immunohistochemical staining of Paraffin embedded Human lymph node tissue, using 1/35 ab121239, shows strong cytoplasmic positivity in reaction center cells.

    参考文献

    This product has been referenced in:
    • Wang YP  et al. Pleomorphic adenoma gene like-2 induces epithelial-mesenchymal transition via Wnt/ß-catenin signaling pathway in human colorectal adenocarcinoma. Oncol Rep 37:1961-1970 (2017). WB, IHC-P ; Human . Read more (PubMed: 28259923) »
    • Guo J  et al. Overexpression of Pleomorphic Adenoma Gene-Like 2 Is a Novel Poor Prognostic Marker of Prostate Cancer. PLoS One 11:e0158667 (2016). Read more (PubMed: 27537362) »

    See all 3 Publications for this product

    ab121239 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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