Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121239 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Western blot - Anti-PLAGL2 antibody (ab121239)
All lanes : Anti-PLAGL2 antibody (ab121239) at 1/250 dilution
Lane 1 : Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2 : Negative control (vector only transfected HEK293T lysate)
Lane 3 : Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)
Immunohistochemical staining of Paraffin embedded Human lymph node tissue, using 1/35 ab121239, shows strong cytoplasmic positivity in reaction center cells.
Anti-PLAGL2 antibody (ab121239) 使用論文
This product has been referenced in:
Wang YP et al. Pleomorphic adenoma gene like-2 induces epithelial-mesenchymal transition via Wnt/ß-catenin signaling pathway in human colorectal adenocarcinoma. Oncol Rep37:1961-1970 (2017).
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Guo J et al. Overexpression of Pleomorphic Adenoma Gene-Like 2 Is a Novel Poor Prognostic Marker of Prostate Cancer. PLoS One11:e0158667 (2016).
Read more (PubMed: 27537362) »