Anti-Placental Protein 14 / Glycodelin A 抗体 (ab123318)

製品の概要

  • 製品名
    Anti-Placental Protein 14 / Glycodelin A antibody
    Placental Protein 14 / Glycodelin A 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Placental Protein 14 / Glycodelin A
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 72-101 of Human Placental Protein 14/ Glycodelin A (NP_001018059.1, NP_002562.2).

  • ポジティブ・コントロール
    • WiDr cell line lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • バッファー
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified through a protein A column, followed by peptide affinity purification.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab123318 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/500. Predicted molecular weight: 18 kDa.

ターゲット情報

  • 機能
    This protein is, quantitatively, the main protein synthesized and secreted in the endometrium from mid-luteal phase of the menstrual cycle and during the first semester of pregnancy.
  • 配列類似性
    Belongs to the calycin superfamily. Lipocalin family.
  • 翻訳後修飾
    At least two differentially glycosylated forms are found. Glycodelin-A (GdA) (amniotic fluid) with contraceptive and immunosuppressive activities and glycodelin-S (Gds) (seminal plasma) whose role is not yet known. The gender-specific glycosylation may serve to regulate key process involved in human reproduction.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Alpha uterine protein antibody
    • gD antibody
    • GdA antibody
    • GdF antibody
    • GdS antibody
    • Glycodelin A antibody
    • Glycodelin antibody
    • Glycodelin F antibody
    • Glycodelin S antibody
    • MGC138509 antibody
    • MGC142288 antibody
    • PAEG antibody
    • PAEP antibody
    • PAEP_HUMAN antibody
    • PEG antibody
    • PEP antibody
    • Placental protein 14 antibody
    • PP14 antibody
    • Pregnancy-associated endometrial alpha-2 globulin antibody
    • Progestagen-associated endometrial protein antibody
    • Progesterone-associated endometrial protein antibody
    see all

画像

  • Anti-Placental Protein 14 / Glycodelin A antibody (ab123318) at 1/100 dilution + WiDr cell line lysates at 35 µg
    Developed using the ECL technique

    Predicted band size : 18 kDa

プロトコール

参考文献

ab123318 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (OV90)
Permeabilization
Yes - 0.25% Triton-X 100 in PBS
Specification
OV90
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative
Formaldehyde
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投稿 Oct 06 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (MDA231 human tumor from Balb/C nude mice)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6
Permeabilization
No
Specification
MDA231 human tumor from Balb/C nude mice
Blocking step
innovex background buster as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 20°C
Fixative
Formaldehyde
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投稿 Oct 05 2016

Application
Western blot
Sample
Human Cell lysate - whole cell (OV90 cells)
Gel Running Conditions
Reduced Denaturing (4-20% Tris Glycine)
Loading amount
50000 cells
Specification
OV90 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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投稿 Aug 31 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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